食品工业科技2017,Vol.38Issue(18):117-122,128,7.DOI:10.13386/j.issn1002-0306.2017.18.023
西番莲果皮中果胶的复合酶法提取工艺研究
Research on the compound enzymatic extraction process of pectin from passionflower peel
摘要
Abstract
Passionflower peel was used as raw materials in the reseach.With enzyme solution pH,hydrolysis time,hydrolysis temperature,enzyme concentration and ratio of liquid to material as single factors,the effects of four kinds of enzyme (cellulase,hemieellulase and lignin enzymes,amylase) on pectin extraction from the passionflower peel were studied.And the enzyme combination consists cellulose,hemicellulase and lignin enzymes were determined.Through L9 (34) orthogonal experiment and response surface test,the enzymatic hydrolysis and enzyme concentration were optimized.the optimal enzymatic hydrolysis conditions were as follows:cellulose enzymes 0.8 g/100 g,hemicellulase 1.2 g/100 g,and lignin 0.2 g/100 g,ratio of liquid to material(mL/g)6∶ 1,pH value 4,extraction lasts for 3.5 h under the temperature of 40 ℃,the pectin gain rate can reach 2.63% ±0.021%.关键词
西番莲/果胶/酶/提取Key words
Passionflower/pectin/enzyme/extraction分类
轻工纺织引用本文复制引用
刘运花,黄苇,郭美媛,黄妙云..西番莲果皮中果胶的复合酶法提取工艺研究[J].食品工业科技,2017,38(18):117-122,128,7.基金项目
广东省级科技计划项目(2014B010102002). (2014B010102002)