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西番莲果皮中果胶的复合酶法提取工艺研究

刘运花 黄苇 郭美媛 黄妙云

食品工业科技2017,Vol.38Issue(18):117-122,128,7.
食品工业科技2017,Vol.38Issue(18):117-122,128,7.DOI:10.13386/j.issn1002-0306.2017.18.023

西番莲果皮中果胶的复合酶法提取工艺研究

Research on the compound enzymatic extraction process of pectin from passionflower peel

刘运花 1黄苇 1郭美媛 2黄妙云2

作者信息

  • 1. 华南农业大学食品学院,广东广州510642
  • 2. 潮州市正一品生物科技有限公司,广东潮州521000
  • 折叠

摘要

Abstract

Passionflower peel was used as raw materials in the reseach.With enzyme solution pH,hydrolysis time,hydrolysis temperature,enzyme concentration and ratio of liquid to material as single factors,the effects of four kinds of enzyme (cellulase,hemieellulase and lignin enzymes,amylase) on pectin extraction from the passionflower peel were studied.And the enzyme combination consists cellulose,hemicellulase and lignin enzymes were determined.Through L9 (34) orthogonal experiment and response surface test,the enzymatic hydrolysis and enzyme concentration were optimized.the optimal enzymatic hydrolysis conditions were as follows:cellulose enzymes 0.8 g/100 g,hemicellulase 1.2 g/100 g,and lignin 0.2 g/100 g,ratio of liquid to material(mL/g)6∶ 1,pH value 4,extraction lasts for 3.5 h under the temperature of 40 ℃,the pectin gain rate can reach 2.63% ±0.021%.

关键词

西番莲/果胶//提取

Key words

Passionflower/pectin/enzyme/extraction

分类

轻工纺织

引用本文复制引用

刘运花,黄苇,郭美媛,黄妙云..西番莲果皮中果胶的复合酶法提取工艺研究[J].食品工业科技,2017,38(18):117-122,128,7.

基金项目

广东省级科技计划项目(2014B010102002). (2014B010102002)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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