食品工业科技2017，Vol.38Issue(18)：173-176,182,5.DOI:10.13386/j.issn1002-0306.2017.18.033
华中枸骨叶抑菌物质提取工艺优化及其抑菌作用研究
Optimizing process of extraction and antibacterial activities of antibacterial substance from Ilex centrochinensis S.Y.Hu leaf
摘要
Abstract
The size of inhibition zone was the index,S.aureus and E.coli were the indicator bacteria,to determine the best extraction method by comparing the bacteriostatic effect of extracts from water extraction,ultrasonic extraction,reflux extraction.Basis on the single-factor experiment,orthogonal experiment was used to optimize the extraction process.The effect of extracts from the optimal process on S.aureus,E.coli,E.aerogenes,P.aeruginosa,S.sonnei,C.albicans was determined.The results showed that the best extraction process was the use of water as solvent,ultrasound extraction,ultrasonic power 250 W,at roomtemperature,material liquid ratio of 1 ∶ 25,extraction for 30 minutes,extracted 2 times.Under these condition of extraction,the diameter of bacteriostatic rings against S.aureus,E.coli were 24.67 mm and 15.67 mm,and the diameter of bacteriostatic rings against E.aerogenes,P.aeruginosa,S.sonnei were 13.67～17.33 mm,and the extraction had no inhibitory effect on C.albicans.关键词
华中枸骨叶/正交实验/抑茵作用Key words
leaf of Ilex centrochinensis S.Y.Hu/orthogonal test/antimicrobial effect分类
轻工纺织引用本文复制引用
杜银香,胡泽华..华中枸骨叶抑菌物质提取工艺优化及其抑菌作用研究[J].食品工业科技,2017,38(18):173-176,182,5.基金项目
国家自然科学基金资助项目(81560703). （81560703）
