食品工业科技2017，Vol.38Issue(18)：215-220,6.DOI:10.13386/j.issn1002-0306.2017.18.041
藜麦麸皮总皂苷的提取纯化工艺研究
Extraction and purification of total saponins from quinoa bran
摘要
Abstract
Solvent reflux method was used for sapouins extraction and the responsive surface methodology was used to optimize the related parameters.Adsorption rate and desorption rate were used as indicators for screening of proper resin for saponins purification and determining of purification techniques.The obtained optimal extraction technique with a ratio(bran to 72％ ethanol)of 1∶20.8,carried out at 72 ℃ for 147 min,and the obtaining rate of saponins reached 1.685％.Compared with HPD600,S-8 and AB-8,the resin D101 showed the highest absorption rate and the highest desorption rate,so it was used for later purification studies.The resuhs suggested that the highest absorption rate for D101 was obtained with a sample concentration of 4 mg/mL,the loading sample was adjusted to pH6 and loading at a velocity of 2 BV/h.The highest desorption rate was obtained when elute with 90％ ethanol at a speed of 2 BV/h.The desorption fraction was condensed and freezing dried,the purity of resulted saponins powder reached 43.6％.The above results will facilitate the high-value application of quinoa bran and the related research work of quinoa saponins.关键词
藜麦麸皮/总皂苷/大孔吸附树脂/溶剂回流提取Key words
quinoa bran/total saponins/macroporous adsorption resin/solvent reflux extraction分类
轻工纺织引用本文复制引用
许效群,赵文婷,苗玲香,霍乃蕊..藜麦麸皮总皂苷的提取纯化工艺研究[J].食品工业科技,2017,38(18):215-220,6.基金项目
山西省重点研发计划重点项目(201603D21102). （201603D21102）
