食品工业科技2017，Vol.38Issue(18)：290-294,299,6.DOI:10.13386/j.issn1002-0306.2017.18.055
链格孢侵染对甜瓜病程相关蛋白活性及基因表达的影响
Effects of activities and gene expression of pathogenesis-related proteins in muskmelon fruit inoculated with Alternaria alternata
摘要
Abstract
Jiashi muskmelon and 86-1 muskmelon species with the characteristics of disease resistance were used as experiment material.This study was aimed to the activities of CHT,GLU and the gene expression rules for CHT and GLU in muskmelon fruits inoculated with A.alternata were analyzed.Jiashi muskmelon and 86-1 muskmelon were inoculated with 20 μL A.alternata spore suspension,inoculation with 20 μL of sterile water as a blank control,the materials were transferred to cold storage at 6～8 ℃,determination of lesion size,the activities of C HT,GLU and the gene expression of CHT and GLU.The results showed that the incidence and the lesion size of Jiashi muskmelon were lower than that of 86-1 muskmelon during the inoculated with A.alternata after 15 days.During the infection period,the activities of CHT,GLU was significantly increased with a trend from rise to decline.The peak of activities for CHT,GLU revealcd of 21 d,18 d.The activities of CHT,GLU in Jiashi melon significantly higher than 86-1 muskmelon Jiashi muskmelon to resist the infection of A.alternaria by enhancing the activity of protease and maintaining the enzyme activity at a higher level.The relative expressions for CHT1,CHT2,GLU were higher in Jiashi muskmelon than 86-1 muskmelon during the whole infection period.The expression levels of CHT1,CHT2 and GLU were significantly higher than 86-1 melon,and the expression level of CHT1,CHT2 and GLU was the highest in storage at 18,18 and 15 days.The relative expression of protein-related protein and gene expression were positively correlated with lesion diameter for 0～21 d.These results suggest that the pathogenesis related proteins play a key role in the early and middle stage of plant pathogen resistance.关键词
甜瓜/链格孢/病程相关蛋白/基因表达Key words
muskmelon/Alternaria alternata/pathogenesis-related proteins/gene expression分类
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张培岭,黄伟,马玲,冯作山,白羽嘉..链格孢侵染对甜瓜病程相关蛋白活性及基因表达的影响[J].食品工业科技,2017,38(18):290-294,299,6.基金项目
新疆维吾尔自治区自然科学基金(青年科学基金,2016D01B020). （青年科学基金,2016D01B020）
