食品与发酵工业2017，Vol.43Issue(9)：28-33,6.DOI:10.13995/j.cnki.11-1802/ts.014688
大豆β-淀粉酶基因在毕赤酵母中的高密度发酵表达
Expression of β-amylase gene from soybean in Pichia pastoris by high cell density fermentation
摘要
Abstract
Soybean β-amylase has significant performance advantages in maltose production.Therefore,in this study,the soybean β-amylase was heterologously expressed in Pichia pastoris.The enzymatic properties of the recombinant β-amylase were examined and found to have no change.To enhance the production of recombinant β-amylase,the methanol/sorbitol co-feeding induction strategy in Pichia pastoris was carried out and the recombinant β-amylase activity reached 310 U/mL in 10 L fermentator,which was 0.36 fold higher than that of strain feeding with methanol alone.These results indicated that heterologous β-amylase expression level could be enhanced by optimizing fermentation condition in Pichia pastoris.关键词
β-淀粉酶/毕赤酵母/异源表达/酶学性质/山梨醇Key words
β-amylase/Pichia pastoris/heterologous expression/enzymatic properties/sorbitol引用本文复制引用
许黎明,成春燕,韦星明,莫祺晖,卢汉浪,杨祥开..大豆β-淀粉酶基因在毕赤酵母中的高密度发酵表达[J].食品与发酵工业,2017,43(9):28-33,6.基金项目
广西自然科学基金面上项目(2015GXNSFAA139087) （2015GXNSFAA139087）
广西自然科学基金青年基金项目(2013GXNSFBA019113) （2013GXNSFBA019113）
广西科学院基本科研业务费资助项目(12YJ25SWS23) （12YJ25SWS23）
