食品与发酵工业2017，Vol.43Issue(9)：209-214,6.DOI:10.13995/j.cnki.11-1802/ts.013452
利用反相高效液相法测定马乳中的乳清蛋白
Determination of whey proteins in mare milk by reversed-phase high-performance liquid chromatography
摘要
Abstract
Whey protein are the main protein in mare milk,but are different in protein composition among different species.In this paper,reserved-phase high-performance liquid chromatography (RP-HPLC) was used to isolate and quantify proteins in mare whey,the main components were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS).The result showed that LYS,α-Ig and β-Ig are the primary components in mare whey proteins.The linear relation of LYS,α-Ig and β-Ig are in the range of 0.1-1.0 (R2 =0.999 3) 0.2-0.8 (R2 =0.996 9) and 0.2-1.0 μg/L(R2 =0.990 3),respectively.The relative standard deviations (RSDs) value of precision and reproducibility tests were all less than 5％,and the spike recovery was in the range of 88.05％-96.26％,86.03％-95.70％ and 91.79％-100.97％,respectively.The concentration of LYS,α-lg and β-lg in mare milk were (0.52-±0.06) 、(3.38 ±0.32) and (2.64 ±0.37) μ.g/L,respectively.关键词
反相高效液相色谱法/马乳/溶菌酶/α-乳白蛋白/β-乳球蛋白Key words
reversed-phase high-performance liquid chromatography (RP-HPLC)/mare milk/lysozyme/α-lactalbumin/β-lactoglobulin引用本文复制引用
李敏婧,杨洁,杨迎春,王红娟,吕岳文,申彤,刘军,阿里木江·阿布都拉..利用反相高效液相法测定马乳中的乳清蛋白[J].食品与发酵工业,2017,43(9):209-214,6.基金项目
国家自然科学基金(31471645) （31471645）
