HPLC-荧光法测定人血浆中伊立替康及其活性代谢产物的浓度OA北大核心CSTPCD
Concentration Determination of Irinotecan and Its Active Metabolite in Human Plasma by HPLC-FLD
目的:建立同时测定人血浆中伊立替康(CPT-11)及其活性代谢产物7-乙基-10-羟基喜树碱(SN-38)浓度的方法.方法:血浆样品经乙腈沉淀蛋白及盐酸酸化后,以喜树碱为内标,采用高效液相色谱-荧光法测定.色谱柱为Waters Luna C18,流动相为0.05 mol/L磷酸二氢钠溶液-乙腈(70:30,V/V,用磷酸调节pH至4.0),流速为1 mL/min,激发波长为380 nm,发射波长为480 nm (CPT-11)、535 nm(SN…查看全部>>
OBJECTIVE:To develop a method for simultaneous determination of irinotecan(CPT-11)and its active metabo-lite 7-ethyl-10-hydroxycamptothecin(SN-38)in human plasma.METHODS:After precipitated by acetonitrile and acidified with hy-drochloric acid,using camptothecin as internal standard,the plasma sample was determined by HPLC-FLD. The determination was performed on Waters Luna C18column with mobile phase consisted of 0.05 mol/L sodium dihydrogen phosphate-aceton…查看全部>>
张晓霈;刘晓明;徐今宁;王爱萍;姜爱雯
河北北方学院药学系,河北张家口075000河北北方学院附属第一医院药剂科,河北张家口075000河北北方学院附属第一医院药剂科,河北张家口075000河北北方学院附属第一医院药剂科,河北张家口075000河北北方学院附属第一医院药剂科,河北张家口075000
医药卫生
高效液相色谱-荧光法伊立替康7-乙基-10-羟基喜树碱血药浓度
HPLC-FLDIrinotecan7-ethyl-10-hydroxycamptothecinPlasma concentration
《中国药房》 2017 (29)
4072-4075,4
河北省医学科学研究重点课题计划(No.20120157)
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