食品科学2017，Vol.38Issue(19)：100-106,7.DOI:10.7506/spkx1002-6630-201719017
乙酸溶胀-挤压提取对罗非鱼真皮胶原蛋白理化性质的影响
Physical and Chemical Properties of Tilapia (Oreochromis niloticus) Dermis Collagen Acetic Acid Swelling and Extrusion
摘要
Abstract
Tilapia skin was pretreated with 0.006 mol/L phosphoric acid at a solid to liquid ratio of 50 g/L for 3 h at 4 ℃ to facilitate the separation of dermis and epidermis.The dermis was treated with 0.035 mol/L acetic acid at a solid to liquid ratio of 100 (g/L) for6 h at 4 ℃ to fully swell it,washed and extruded to obtain collagen.The results showed that tilapia dermis collagen contained 7.9％ of hydroxyproline,and the collagen yield was as high as 27.6％ (on a dry basis).The collagen mainly consisted of α1,α2 and β polypeptide chains according to sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis,with maximum UV absorbance at 230 nm,complying with the characteristics of type Ⅰ collagen.The attenuated total reflectance Fourier transform infrared (ATR FT-IR) spectrum proved the presence of the triple-helix structure of collagen.The thermal denaturation temperature and shrinkage temperature of the collagen were 29.2 ℃ and 55.0 ℃,respectively.The study provides an alternative approach to the extraction of high-quality collagen and consequently efficient utilization of fish processing byproducts.关键词
罗非鱼皮/真皮/表皮/溶胀/提取Key words
tilapia skin/dermis/epidermis/swelling/extraction分类
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鲍虹蕾,杨敏,刘文文,何沁峰,崔改泵,陈伟,胡建恩,武龙..乙酸溶胀-挤压提取对罗非鱼真皮胶原蛋白理化性质的影响[J].食品科学,2017,38(19):100-106,7.基金项目
国家海洋局海洋公益性行业科研专项(201505030-3) （201505030-3）
辽宁省大学生创新创业训练计划项目(201510158000006) （201510158000006）
