食品科学2017，Vol.38Issue(19)：164-170,7.DOI:10.7506/spkx1002-6630-201719026
细胞水平评价沙棘粕醇提物的抗衰老功效
Anti-Aging Effects of Sea Buckthorn Seed Extracts
摘要
Abstract
In this research,sea buckthorn seed extracts (SBSE) were obtained by the extraction of sea buckthorn seed residues using ethanol.SBSE were separated into two fractions of different polarities:the upper ethyl acetate phase and the bottom aqueous phase.The crude extract and its fractions were tested for bioactive components,chemical and cellular antioxidant properties including scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radicals and catalase activity,and their effects on reactive oxygen (ROS),matrix metalloproteinase-1 (MMP-1)and type Ⅰ collagen (COL Ⅰ) contents in the culture supernatants of human skin fibroblast cells were examined.The results showed that flavonoids,proanthocyanidins and total phenolics were the major bioactive compounds in the crude extract and its fractions.All these samples could strongly scavenge DPPH free radical.The two fractions but not the crude extract showed potent hydroxyl radical scavenging activity.All three samples in the concentration range of 0.05-5.00 mg/mL showed no strong cytotoxicity to human skin fibroblast cells and the cell viability was above 80％ in the presence of each sample.Stimulation with the samples significantly increased catalase activity and reduced ROS content,and the crude extract showed the strongest catalase activity and lowest ROS level among these samples.Furthermore,cellular MMP-1 contents were significantly decreased by SBSE,and COL Ⅰ contents were increased to the maximum.Therefore,SBSE had potential anti-aging effect.关键词
沙棘粕/粗提物/乙酸乙酯层/萃余水层/抗衰老Key words
sea buckthorn seed residues/crude extracts/ethyl acetate phase/aqueous phase/anti-aging potential分类
轻工纺织引用本文复制引用
张佳婵,史豆豆,王昌涛,李萌,赵丹,王成涛,孙宝国..细胞水平评价沙棘粕醇提物的抗衰老功效[J].食品科学,2017,38(19):164-170,7.基金项目
国家自然科学基金面上项目(31571801) （31571801）
