食品科学2017，Vol.38Issue(19)：171-176,6.DOI:10.7506/spkx1002-6630-201719027
金银花叶黄酮对衰老模型小鼠的体内抗氧化作用
In Vivo Antioxidant Activity of Honeysuckle Leaf Flavonoids in Aging Mice
摘要
Abstract
Objective:To study the effect of honeysuckle leaf flavonoids on the antioxidant defense system in various tissues of aging mice.Methods:An aging mouse model was established by subcutaneous injection of D-galactose in the neck and back,and the aging mice were gavaged with different doses (50,100 and 200 mg/(kg·d)) of honeysuckle leaf flavonoids.Changes in body weight were observed over the 30-day administration period,and liver,brain,kidney,spleen and heart indexes and the contents of malondialdehyde (MDA) and glutathione (GSH) and the activities of total antioxidant capacity (T-AOC),superoxide dismutase (SOD),glutathione peroxidase (GSH-Px) and catalase (CAT) in the liver,brain,kidney,heart and serum of mice were assessed.Results:Compared with the control group,the body weight and organ indexes of mice in the model group decreased significantly,T-AOC,SOD,GSH-Px and CAT activities and GSH content in the serum and organs decreased,and MDA content increased,with significant differences being observed for most of these parameters (P ＜ 0.05),indicating successful construction of aging mice model.Compared with the model group,body weight,organ indexes and T-AOC,SOD,GSH-Px and CAT activities as well as GSH content in the serum and organ tissues were improved for all dose groups;on the other hand,MDA content was decreased.The most significant effect was obtained at the high dose.Conclusion:Honeysuckle leaf flavonoids can effectively improve the antioxidant defense capacity of aging mice through a mechanism related to the increased activities of antioxidant enzymes and the reduced contents of protective substances and lipid peroxide.关键词
金银花叶/黄酮/衰老模型小鼠/D-半乳糖/抗氧化能力Key words
honeysuckle leaf/flavonoids/aging mice/D-galactose/antioxidant capacity分类
轻工纺织引用本文复制引用
罗磊,张冰洁,关宁宁,马丽苹,朱文学..金银花叶黄酮对衰老模型小鼠的体内抗氧化作用[J].食品科学,2017,38(19):171-176,6.基金项目
国家自然科学基金联合基金项目(U1304330) （U1304330）
河南省高校科技创新团队计划项目(17IRTSTHN016) （17IRTSTHN016）
