食品科学2017，Vol.38Issue(20)：1-5,5.DOI:10.7506/spkx1002-6630-201720001
红鳍东方纯组织蛋白酶L表达载体的构建及表达结果验证
Construction and Verification of Expression Vector for Takifugu rubripes Cathepsin L Gene
摘要
Abstract
Takifugu rubripes has a delicious taste.To date,a series of umami peptides have been separated and identified from T.rubripes,but their formation mechanism is not yet clear.Cathepsin L degrades myofibrillar protein and troponin during muscle degradation and thus plays an important role in the flavor formation.This study aimed to elucidate the possible mechanism of flavor peptide formation.We obtained a 1 336 bp sequence of cathepsin L gene from the NCBI database,and extracted RNA from the muscle of T.rubripes,and then reverse-transcribed it into cDNA.PET-28a (+) as the expression vector and E.coli BL21 as engineering bacteria was used to clone and express the cathepsin L gene.Recombinant cathepsin L was obtained with a molecular mass of 36 kD,and the E.coli expression system was also successfully validated.This study can provide useful information for further studies on enzymatic characteristics and of cathepsin L and its role in taste formation in T.rubripes.关键词
红鳍东方鲀/组织蛋白酶L/呈味肽/大肠杆菌表达系统Key words
Takifugu rubripes/cathepsin L/flavor peptide/E.coli expression system分类
轻工纺织引用本文复制引用
李妍钰,汤奇龙,张鲁嘉,谢静莉,王文利,刘源..红鳍东方纯组织蛋白酶L表达载体的构建及表达结果验证[J].食品科学,2017,38(20):1-5,5.基金项目
国家自然科学基金青年科学基金项目(31622042 （）
31371790 （）
31271900) （）
“十三五”国家重点研发计划重点专项(2016YFD040083) （2016YFD040083）
