食品科学2017，Vol.38Issue(20)：6-11,6.DOI:10.7506/spkx1002-6630-201720002
真菌灰树花菌丝体转录组测序及分析
Transcriptome Sequencing and Analysis of Grifola frondosa Mycelia
摘要
Abstract
The exopolysaccharide of Grifola frondosa possesses anti-tumor,anti-HIV,and anti-oxidant effects.In order to investigate the genetic basis for polysaccharide synthesis by G.frondosa,the transcriptome of G.frondosa was sequenced by high-throughput sequencing technology.A total of 74 575 910 reads and 10.4 G data were generated,which formed 18 077 Unigenes after de novo splicing.A total of 11 651 Unigenes were annotated in the Nr database.Among them,G.frondosa transcriptome had the most similar sequence (18.74％) to M.tumefaciens.A total of 8 332 Unigenes were annotated in the GO database,which were divided into 3 major categories:molecular composition,molecular function and biological processes,including 59 branches.Compared with the KEGG database,5 200 Unigenes were annotated which belonged to 376 metabolic pathways.A total of 1 155 simple sequence repeat (SSR) loci were found in 18 077 Unigenes,among which the highest repeat was single nucleotide whereas the repetition of hexanucleotide was the least,and the frequency of A/T was the highest.In this study,115 unigenes which have been annotated in the database of KEGG were related to the biosynthesis of polysaccharide by G.frondosa.These annotated Unigenes and the information about them can lay the foundation for further studies on polysaccharide metabolism pathways and related functional genes in G.frondosa.关键词
灰树花/菌丝体/转录组测序/功能注释Key words
Grifola frondosa/mycelium/transcriptome sequencing/functional annotation分类
生物科学引用本文复制引用
聂文强,吴天祥,钟敏,芦红云..真菌灰树花菌丝体转录组测序及分析[J].食品科学,2017,38(20):6-11,6.基金项目
国家自然科学基金地区科学基金项目(31460537) （31460537）
