食品科学2017，Vol.38Issue(20)：12-19,8.DOI:10.7506/spkx1002-6630-201720003
李果实褐腐病病原菌产生细胞壁降解酶条件优化及其致病机理
Optimization of Culture Conditions of Monilinia fructicola for the Production of Cell Wall Degrading Enzymes and Their Involvement in Brown Rot Pathogenesis of Postharvest Plums
摘要
Abstract
The aims of this study were to optimize the culture conditions of Monilinia fructicola for the production of cell wall degrading enzymes (CWDE) and to clarify the role of the culture supernatant obtained as crude CWDE in the pathogenesis of brown rot caused by Moniliniafructicola in postharvest plums.The optimization of the culture conditions was carried out using one-factor-at-a-time and orthogonal array design methods.Besides,the pathogenesis was investigated by wounding and inoculating plum fruits with the crude CWDE and observing the incidence of brown rot.The results showed that the optimal culture conditions were as follows:culture time,6 d;pH,6.0;3.5％ sucrose as carbon source;and 2.5％ KNO3 as nitrogen source.The crude enzymes produced by M.fructicola could cause brown rot of plum fruits.In addition,the activities of polygalacturonase (PG),pectin methylgalacturonase (PMG) in the fruits inoculated with the crude enzymes were enhanced,thereby leading to the conversion of insoluble protopectin to soluble pectin and cellulose reduced cellulose content and consequently accelerating the softening and rotting process.关键词
褐腐病/细胞壁降解酶/李果实/致病机理Key words
brown rot/cell wall degrading enzymes/plum/pathogenesis分类
农业科技引用本文复制引用
张婕,凡先芳,姚世响,邓丽莉,曾凯芳..李果实褐腐病病原菌产生细胞壁降解酶条件优化及其致病机理[J].食品科学,2017,38(20):12-19,8.基金项目
国家公益性行业(农业)科研专项(201303075) （农业）
重庆市研究生科研创新项目(CYS16071) （CYS16071）
