食品科学2017，Vol.38Issue(20)：27-33,7.DOI:10.7506/spkx1002-6630-201720005
基于16S rDNA高通量测序方法比较新疆西北部地区乳品中微生物的多样性
Application of 16S rDNA High-Throughput Sequencing for Comparative Study of the Microbial Diversity of Dairy Products from Western and Northern Xinjiang, China
摘要
Abstract
This study aimed to fully demonstrate the microbial diversity in dairy products from Xinjiang,China and to compare the microbial community composition of raw and fermented milk from different animal species.The composition and structure of bacterial communities in bovine milk,camel milk,mare's milk,caprine milk,fermented cow milk,fermented camel milk and koumiss from Kizilsu Kirghiz and Tacheng regions of Xinjiang were analyzed based on high-throughput sequencing of the V4-V5 region of the 16S rDNA.A total of 539 557 effective sequences and 379 OTUs were obtained.The results showed that the Shannon-Wiener diversity index for raw milk was higher than that for fermented milk.The microbiota of raw and fermented milk were significantly different.Two bacterial phyla,Firmicutes and Proteobacteria,dominated the microbiota of all seven dairy products,and Proteobacteria dominated the microbiota of raw milk,while Firmicutes was the predominant phylum in fermented milk.At the genus level,Pseudoraonas was dominant in cow milk,Escherichia-Shigella was the major bacterial population in camel milk,Leuconostoc was the most abundant bacteria in mare's milk,Lactococcus was the dominant bacteria in goat milk samples,and Lactobacillus dominated the microbiota of yogurt,camel yogurt and koumiss.The microbiota in raw and fermented milk from different animals were significantly different,and the abundances of environmental bacterial and pathogenic bacteria (or conditional pathogenic bacteria) in raw milk were highest.The results of this study would provide preliminary data of the microbial diversity in dairy products to assess their effect on the health of Xinjiang minorities.关键词
乳品/微生物多样性/高通量测序技术Key words
dairy product/microbial diversity/high-throughput sequencing分类
生物科学引用本文复制引用
张敏,张艳,黄丽丽,刘忆冬,周红,倪永清..基于16S rDNA高通量测序方法比较新疆西北部地区乳品中微生物的多样性[J].食品科学,2017,38(20):27-33,7.基金项目
新疆生产建设兵团科技攻关与成果转化计划项目(2015AC003) （2015AC003）
