食品科学2017，Vol.38Issue(20)：139-145,7.DOI:10.7506/spkx1002-6630-201720020
血管紧张素转换酶抑制肽RLSFNP脂质体的制备工艺优化
Optimization of Preparation of Angiotensin Converting Enzyme (ACE) Inhibitory Peptide RLSFNP Liposomes
摘要
Abstract
The objective of the present study was to optimize the preparation of liposomes incorporating the angiotensin converting enzyme (ACE) inhibitory peptide Arg-Leu-Ser-Phe-Asn-Pro (RLSFNP) using combination of one-factor-at-a-time method and response surface methodology.The response variable was the entrapement efficiency.The optimum preparation conditions were determined as follows:soy lecithin,120.0 mg;cholesterol,24.6 mg;RLSFNP,15.3 mg;ether,15 mL;PBS buffer (pH 7.4),5.8 mL;and ultrasonication time,5.2 rain.Under these conditions,the average diameter,potential and entrapement efficiency of liposomes were 168 nm,-31.2 mV and (67.5 ± 0.8) ％,respectively.The liposomes could sustainably release RLSFNP.关键词
血管紧张素转换酶抑制肽/脂质体/包封率/生物利用率Key words
angiotensin converting enzyme (ACE) inhibitory peptides/liposomes/entrapement efficiency/bioavailability分类
轻工纺织引用本文复制引用
郭宇星,薛逸秋,姜潇潇,吴振,曾小群,孙杨赢,潘道东..血管紧张素转换酶抑制肽RLSFNP脂质体的制备工艺优化[J].食品科学,2017,38(20):139-145,7.基金项目
国家自然科学基金面上项目(31571852 （）
31471598 （）
31671869 （）
31601487) （）
江苏省自然科学基金项目(BK20151544) （BK20151544）
2017年江苏省第五期“333工程”培养资金资助项目(BRA2017450) （BRA2017450）
