食品科学2017，Vol.38Issue(20)：154-160,7.DOI:10.7506/spkx1002-6630-201720022
沙棘渣制备微品纤维素的酶解条件优化
Optimization of Enzymatic Hydrolysis Conditions for Preparation of Microcrystalline Cellulose from Seabuckthorn Pomace
摘要
Abstract
Seabuckthom pomace is rich in phenolic acid and is a byproduct produced during the processing of seabuckthorn pulp.However,the astringency and bitterness of seabuckthorn pomace hinder its utilization in the feed industry.The preparation of microcrystalline cellulose from seabuckthorn pomace is a potential solution to this problem.Therefore,in this study,the crude cellulose extracted from seabuckthorn pomace was hydrolyzed with commercial cellulase S10041 to obtain microcrystalline cellulose.Eight processing parameters were investigated,namely solid-to-solvent ratio,enzyme dosage,hydrolysis time,temperature,buffer pH,centrifugal rotational speed,drying temperature,and comminution degree of cellulose.The significant factors were selected and optimized using one-factor-at-a-time method,Plackett-Burman design,steepest ascent path design and Box-Behnken design combined with response surface methodology.The prepared microcrystalline cellulose was structurally elucidated.The results showed that the degree of polymerization of seabuckthorn microcrystalline cellulose was 355 ± 1.02 under the optimal follows:ratio of buffer solution to cellulose,49∶1 (mL/g);enzyme dosage,68 U/mL;hydrolysis time,1.3 h;and centrifugal rotational speed,3 640 r/min,which was close to that of cotton microcrystalline cellulose.The analysis of variance showed that the four selected factors had independent influences on the degree of polymerization but their interactions had no significant effect on the response (P =0.10).The scanning electron micrograph revealed that the surface structure of seabuckthorn microcrystalline cellulose was more porous as compared to cotton microcrystalline cellulose.The infrared spectra of two microcrystalline celluloses revealed that similar functional groups existed.关键词
沙棘渣/微晶纤维素/纤维素酶Key words
seabuckthorn pomace/microcrystalline cellulose/cellulase分类
轻工纺织引用本文复制引用
盛文军,毕阳,冯丽丹,李霁昕,韩舜愈,李敏..沙棘渣制备微品纤维素的酶解条件优化[J].食品科学,2017,38(20):154-160,7.基金项目
国际科技合作重点计划项目(2014DFR31230) （2014DFR31230）
