食品科学2017，Vol.38Issue(20)：185-190,6.DOI:10.7506/spkx1002-6630-201720026
花脸香蘑菌丝体多糖提取条件优化及其体外免疫活性
Extraction and Immune Modulatory Activities in Vitro of Polysaccharides from Lepista sordida Mycelia
摘要
Abstract
This study aimed to improve the extraction yield and evaluate the immune modulatory activity of polysaccharides of Lepista sordida mycelia.Optimization of the extraction conditions was carried out using one-factor-at-a-time and orthogonal array design methods.The immune modulatory effect of the polysaccharides extracted was evaluated by measuring cell survival,TNF-α and IL-6 secretion,the percentage of neutral red phagocytosis and the expression levels of Toll-like receptor (TLR) TLR2 and TLR4 when mouse Ana-1 macrophages were exposed to different concentrations of the polysaccharides.The optimal extraction conditions were determined as follows:extraction temperature,65 ℃;extraction time,2.5 h;solvent-to-solid ratio,40∶1 (mL/g),leading to an extraction yield of 15.88％.In the concentration range of 0.05-1.6 mg/mL,the polysaccharides could promote the proliferation of macrophages,TLR2 and TLR4 expression,the phagocytosis of neutral red and TNF-α and IL-6 production;the most effective concentration was 1.6 mg/mL.These data illustrated that the polysaccharides possessed good immune modulatory activity.关键词
花脸香蘑菌丝体/多糖/提取工艺/正交试验/免疫活性Key words
Lepista sordida mycelium/polysaccharides/extraction/orthogonal array design/immune modulatory activities分类
农业科技引用本文复制引用
胡欣蕾,田雪梅,李文香,孙亚男,张欣,于戈,李铭..花脸香蘑菌丝体多糖提取条件优化及其体外免疫活性[J].食品科学,2017,38(20):185-190,6.基金项目
山东省科技发展计划项目(2014GNCl13007) （2014GNCl13007）
山东省现代农业产业技术体系建设经费资助项目(SDAIT-07-07) （SDAIT-07-07）
