食品科学2017，Vol.38Issue(20)：203-208,6.DOI:10.7506/spkx1002-6630-201720029
分子印迹固相萃取-高效液相色谱法检测奶粉中的双氰胺
Molecularly Imprinted Solid-Phase Extraction Coupled with High Performance Liquid Chromatography for the Detection of Dicyandiamide in Milk Powder
摘要
Abstract
A novel molecularly imprinted polymer was developed by a one-step polymerization using dicyandiamide as template,methacrylic acid as functional monomer,ethylene glycol dimethylacrylate as cross-linker,and azobisisobutyronitdle as initiator.The physicochemical properties of the prepared material were characterized by scanning electron microscopy,Fourier transform infrared spectroscopy,and ultraviolet spectroscopy.A molecularly imprinted solid phase extraction coupled with high performance liquid chromatography method was developed using this material to detect dicyandiamide in real milk powder samples.The linear range of this method was from 0.01 to 2.00 mg/L,with a correlation coefficient (R2) equal to 0.999 4,which had a good linear relationship.The limit of detection (LOD) was 0.57 × 10-2 mg/L (RSN=3).The recovery was ranged from 102.5％ to 104％,and the relative standard deviation was between 0.21％ and 0.86％.关键词
双氰胺/分子印迹聚合物/固相萃取/高效液相色谱Key words
dicyandiamide/molecularly imprinted polymers (MIP)/solid-phase extraction/high performance liquid chromatography (HPLC)分类
轻工纺织引用本文复制引用
刘慧琳,倪天鸿,周凯文,王静..分子印迹固相萃取-高效液相色谱法检测奶粉中的双氰胺[J].食品科学,2017,38(20):203-208,6.基金项目
国家自然科学基金青年科学基金项目(31501559) （31501559）
国家自然科学基金面上项目(31571940) （31571940）
北京市教委一般项目(201610011004) （201610011004）
