食品科学2017，Vol.38Issue(20)：235-241,7.DOI:10.7506/spkx1002-6630-201720034
QuEChERS法-液相色谱-串联质谱法测定蔬菜中7种农药及其代谢物的残留量
Determination of 7 Pesticide and Metabolite Residues in Vegetables Using QuEChERS and Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A method was developed for the simultaneous determination of 7 pesticide and metabolite residues in vegetables by employing quick,easy,cheap,effective,rugged and safe (QuEChERS) extraction coupled with liquid chromatography triple quadrupole mass spectrometry (LC-MS/MS).Samples were extracted with acetonitrile,and then the extract was purified by dispersive solid phase extraction (SPE) using graphite carbon black (GCB) and C18 sorbent.The analytes were monitored by liquid chromatography-tandem triple quadrupole mass spectrometry in the dynamic multiple reaction monitoring (DMRM) mode,and quantification was carried out by the external standard method with matrix-match standard solution.The recoveries at spiked levels of 10 and 20 μg/kg were in the range from 71.9％ to 117.8％ and the precision,expressed as relative standard deviation (RSD,n=3),ranged from 0.8％ to 9.6％ under the selected conditions.The limits of quantification (LOQs) were in the range of 0.2-10 μg/kg.The method proved to be simple,fast,sensitive and useful for the analysis of the 7 pesticides and their metabolite residues in vegetables.关键词
液相色谱-串联质谱/蔬菜/农药及其代谢物Key words
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/vegetables/pesticides and metabolites分类
农业科技引用本文复制引用
王建华,葛敏敏,张慧丽,林洪,汤志旭,李立..QuEChERS法-液相色谱-串联质谱法测定蔬菜中7种农药及其代谢物的残留量[J].食品科学,2017,38(20):235-241,7.基金项目
公益性行业(质检)科研专项(201410177 （质检）
201210012) （）
