食品科学2017，Vol.38Issue(20)：242-247,6.DOI:10.7506/spkx1002-6630-201720035
基于单链抗体的呋喃唑酮酶联免疫检测方法的建立
Establishment of Enzyme-Linked Immunosorbent Assay Method for Detecting Furazolidone Based on Single Chain Fragment Antibody
摘要
Abstract
Aim:This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) method for detecting the residues of furazolidone (FZD) in animal food.Method:The indirect competitive ELISA method based on single chain fragment antibody was established.Result:The optimal antigen mass concentration was 2 μg/mL,and the optimal antibody dilution ratio was 1∶500,and the optimal reaction time of primary antibody,the optimal reaction time of secondary antibody and the optimal reaction time of TMB were 60 min,45 min,and 20 min,respectively.The good linearity was seen in the range of 10-100 ng/mL of FZD,with the IC50 value being 13.01 ng/mL,and the lowest detection limit (LOD) being 1.28 ng/mL,and the recovery rates being 73.38％-84.52％.Conclusion:Compared with the monoclonal antibody against FZD,the detection kit based on single chain fragment antibody displayed wider detection range,higher sensitivity,better specificitv and detection stability.关键词
呋喃唑酮/单链抗体/酶联免疫检测Key words
furazolidone/single chain fragment antibody/enzyme-linked immunosorbent assay分类
轻工纺织引用本文复制引用
陈倩,陈荫楠,陈东海,林海虹,石贤爱..基于单链抗体的呋喃唑酮酶联免疫检测方法的建立[J].食品科学,2017,38(20):242-247,6.基金项目
国家海洋局海洋公益性行业科研专项(201205022-3) （201205022-3）
福建省科技重大专项(2013NZ0003) （2013NZ0003）
福建省海洋与渔业厅重点项目(闽海渔合同[2010]2-27号) （闽海渔合同[2010]2-27号）
