食品科学2017，Vol.38Issue(20)：262-267,6.DOI:10.7506/spkx1002-6630-201720038
基质辅助激光解析飞行质谱法检测空肠弯曲菌及李斯特菌
Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry for the Detection of Campylobacter jejuni and Listeria monocytogenes
摘要
Abstract
Objective:To establish a matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) method to detect Campylobacterjejuni and Listeria monocytogenes in environmental samples from a chicken farm and samples from commercial live chickens using VITEK MS.Methods:The suspicious colonies of both bacteria were separated from the samples,and then suspended in matrix solutions consisting of mixtures of different proportions of ethanol,acetonitrile and water before identification by MALDI-TOF-MS.The effect of matrix solution composition on the bacterial detection was evaluated by examining the differences in characteristic peak value and relative abundance.Results:Significantly different matrix solutions were needed for the detection of Campylobacter jejuni and Listeria monocytogenes;the former did not require cracking by formic acid and was sensitive to trifluoroacetic acid (TFA),but the latter were just the opposite.A 30％ water content in matrix solution (V/V) could avoid the lack of characteristic peak and enhance the detection rate.Conclusion:Control of the water content in matrix solution,TFA concentration and matrix concentration can enhance the number and relative abundance of characteristic peaks,and detection rate.The optimized matrix solution enables the rapid identification of foodbome pathogens,which was able to improve the timeliness of disease prevention and food-borne risk monitoring.关键词
VITEK MS微生物检测系统/空肠弯曲菌/单核细胞性李斯特菌/基质液配比/微生物质谱检测方法Key words
VITEK MS microbe identification system/Campylobacter jejuni/Listeria monocytogenes/matrix solution composition/mass spectrometric detection of microbes分类
医药卫生引用本文复制引用
屠博文,吉俊敏,杜强,黎俊宏,唐宏兵,熊吉滨,韩晓冬..基质辅助激光解析飞行质谱法检测空肠弯曲菌及李斯特菌[J].食品科学,2017,38(20):262-267,6.基金项目
江苏省自然科学基金项目(BK20150250) （BK20150250）
江苏省卫生计生委科研项目(Y2015016) （Y2015016）
常州市科技项目(CE20165042) （CE20165042）
