食品科学2017，Vol.38Issue(20)：327-333,7.DOI:10.7506/spkx1002-6630-201720049
超高效液相色谱-串联四极杆质谱快速筛查豆芽中51种抗生素类药物的残留
Rapid Screening of 51 Antibiotic Residues in Bean Sprouts by UPLC-MS/MS
孙晓冬 1郝杰 1毛婷 1史娜 1姜洁1
作者信息
- 1. 北京市食品安全监控和风险评估中心,北京 100041
- 折叠
摘要
Abstract
A rapid method was established for screening 51 antibiotic residues in bean sprouts using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) after a rapid and high throughput pretreatment.Samples were extracted with acetonitrile and the extract was cleaned up by quick,easy,cheap,effective,rugged,and safe (QuEChERS) extraction.The target compounds were separated on BEH C18 UPLC column with gradient elution,monitored using positive electrospray ionization in the multiple-reaction monitoring (MRM) mode and quantitated by the external standard method.The results showed that the limits of quantity (LOQs) for 51 antibiotics were between 0.5 and 5 μg/kg.Average recoveries at 3 different spiked levels were 62.93％-118.69％,and the precision values expressed as relative standard deviations (RSDs) were 0.29％-9.72％.This method proved to be a rapid,high throughput,multi-component screening method for antibiotic residues in bean sprouts.关键词
豆芽/超高效液相色谱-串联四极杆质谱/抗生素类药物/快速筛查Key words
bean sprout/ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)/antibiotics/rapid screening分类
轻工纺织引用本文复制引用
孙晓冬,郝杰,毛婷,史娜,姜洁..超高效液相色谱-串联四极杆质谱快速筛查豆芽中51种抗生素类药物的残留[J].食品科学,2017,38(20):327-333,7.
