摘要
Abstract
An effective method was established for simultaneous determination of vitamin B6,nicotinamide,lemon yellow,carmine,caffeine,allura red and benzoic acid in functional drinks by high performance liquid chromatography (HPLC).The effective separation was achieved with Agilent Eclipse Plus C18 column using methanol and 0.02mol/L NH4Ac (pH=4.5)as mobile phase and gradient elution.Samples were detected by a diode array detector at 230,243,254 and 280 nm.In this method,vitamin B6,nicotinamide,caffeine and benzoic acid exhibited excellent linearity over the range of 0.1 ～ 70mg/L,carmine and allura red exhibited excellent linearity over the range of 0.1 ～ 7mg/L,while lemon yellow exhibited excellent linearity over the range of 0.5 ～ 7mg/L and correlation coefficients all above 0.999 for 7 components.The detection limits ranged from 0.1 to 0.5mg/L,which met the requirements of the national standard.The average recoveries for three pigments in a functional drink were in the range of 92.4％ ～ 109.5％ with relative standard deviations (n=6) of 0.33％ ～ 4.92％.The method was convenient,accurate and suitable for simultaneous determination of 7 pigments in functional drinks.关键词
高效液相色谱/功能饮料/VB6/烟酰胺/柠檬黄/胭脂红/咖啡因/诱惑红/苯甲酸Key words
high performance liquid chromatography/functional drink/vitamin B6/nicotinamide/lemon yellow/carmine/caffeine/allura red/benzoic acid分类
轻工纺织
