食品与发酵工业2017，Vol.43Issue(10)：88-95,8.DOI:10.13995/j.cnki.11-1802/ts.014537
产高分子量木聚糖酶细菌的筛选及产酶条件优化
Screening of high molecular weight xylanase-producing bacteria and optimization of fermentation conditions
摘要
Abstract
55 Xylanase-producing bacteria from the different sources of soil samples through the transparent circle method a high molecular weight xylanase-producing bacteriaby liquid fermentation and SDS-PAGE zymogramanalysis.Through single factor experiment and response surface analysis,the factors affecting the enzyme production fermentation optimizedthe best enzyme production fermentation conditions:carbon source (corn cob powder)0.8％,nitrogen source (beef paste) 1％,initial pH value of 7.2,culture temperature 30 ℃,rotational speed of 158 r/min,of 2％,the fluid volume 50 mL.Under the optimized condition,the enzyme activity reached 44.12 U/mL after fermentation,which 4.36 times higher than before optimization.关键词
高分子量木聚糖酶/细菌菌株/液态发酵/响应面优化Key words
high molecular weight xylanase/bacterial strains/liquid fermentation/the response surface optimization引用本文复制引用
王中月,滕超,汤回花,李秀婷..产高分子量木聚糖酶细菌的筛选及产酶条件优化[J].食品与发酵工业,2017,43(10):88-95,8.基金项目
国家自然科学基金(31671793 （）
1201449) （）
