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微生物扩增子高通量测序技术在水产品加工与贮藏中的应用

王伟 冷凯良 刘均忠 郝建华 孙谧

食品与发酵工业2017,Vol.43Issue(10):263-268,6.
食品与发酵工业2017,Vol.43Issue(10):263-268,6.DOI:10.13995/j.cnki.11-1802/ts.014760

微生物扩增子高通量测序技术在水产品加工与贮藏中的应用

High-throughput microbial rRNA amplicon sequencing in the study of processing and storage of aquatic food products

王伟 1冷凯良 2刘均忠 1郝建华 1孙谧2

作者信息

  • 1. 中国水产科学研究院黄海水产研究所,农业部极地渔业开发重点实验室,山东青岛,266071
  • 2. 青岛海洋科学与技术国家实验室,海洋药物与生物制品功能实验室,山东青岛,266235
  • 折叠

摘要

Abstract

Food microbiology has been revolutionized by the advances in molecular genetic technology of microbial ecology.Novel microbial detection methods have been applied in the study of processing and storage of aquatic food products.The microbial diversity in the sample to be studied could be detected by high-throughput sequencing (HTS) approaches as culture-independent analyses.The aim of this review is to give a brief introduction of the microbial amplicon HTS technology,to present a survey of recent aquatic food investigations via 16S rRNA amplicon HTS and to illustrate the potential of this approach for a valuable tool in the better characterization of aquatic foods and their processing and storage.

关键词

扩增子高通量测序/16S rRNA基因/水产品加工与贮藏/微生物检测

Key words

shigh-throughput amplicon sequencing/16S rRNA gene/processing and storage of aquatic products/microbe detection

引用本文复制引用

王伟,冷凯良,刘均忠,郝建华,孙谧..微生物扩增子高通量测序技术在水产品加工与贮藏中的应用[J].食品与发酵工业,2017,43(10):263-268,6.

基金项目

中国水产科学研究院基本科研业务费项目(2016HY-ZD0902) (2016HY-ZD0902)

青岛海洋科学与技术国家实验室鳌山科技创新计划项目(2016ASKJ14) (2016ASKJ14)

食品与发酵工业

OA北大核心CSCDCSTPCD

0253-990X

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