现代食品科技2017，Vol.33Issue(10)：7-12,6.DOI:10.13982/j.mfst.1673-9078.2017.10.002
猫豆乙醇提取物对3T3-L1脂肪细胞糖脂代谢的影响
Effects of Mucuna pruriens (Linn) Ethanol Extracts on Glucose and Lipid Metabolism in 3T3-L1 Adipocytes
摘要
Abstract
To investigate the effect of Mucuna pruriens (Linn) ethanol extracts (MPEE) on glucose and lipid metabolism in insulin resistance 3T3-L 1 adipocytes.The insulin resistance 3T3-L1 adipocyte model was induced by DMEM medium containing high concentration of glucose and insulin.The levels of glucose consumption,free fatty acid (FFA),glycerol,cellular triglyceride(TG),tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the culture medium were determined by commercial assay kits after the cells treated with different concentrations of MPEE for 24 h.The mRNA expression levels of glucose transporter-4 (GLUT-4),hormone-sensitive triglyceride lipase(HSL) and inflammation related factors [including TNF-α,IL-6,monocyte chemotactic protein-1 (MCP-1) and C-reaction protein (CRP)] in cells were determined by Quantitative real-time PCR (qRT-PCR) assay.MPEE could enhance the glucose consumption,decrease the cellular TG levels and reduce the releasing of FFA and glycerol in insulin resistance cells.MPEE also reduced the secretion of TNF-α and IL-6,and reduced the mRNA expressions of inflammation mediators (including TNF-α,IL-6,MCP-1 and CRP) in model cells.In addition,MPEE was able to up-regulate the mRNA levels of GLUT-4,and reduce the mRNA levels of HSL in insulin resistance cells.These results suggested that the MPEE treatment was able to regulate the glucose and lipid metabolism to against the insulin resistance via increasing the glucose consumption,enhancing the TG lipolysis,and reducing the releasing of FFA and glycerol in 3T3-L1 adipocytes.MPEE treatment also down-regulated the inflammation levels by reducing the secretion of inflammatory mediators.关键词
猫豆乙醇提取物/3T3-L1/胰岛素抵抗/糖脂代谢Key words
Mucuna pruriens ethanol extracts/3T3-L1/insulin resistance/glucose and lipid metabolism引用本文复制引用
宋家乐,钱波,桂中玉,朴建荣,周燕园..猫豆乙醇提取物对3T3-L1脂肪细胞糖脂代谢的影响[J].现代食品科技,2017,33(10):7-12,6.基金项目
广西壮族自治区教育厅中青年教师基础能力提升项目(KY2016YB328、KY2016YB321) （KY2016YB328、KY2016YB321）
广西壮族自治区“大学生创新创业训练计划”资助项目(201510601076) （201510601076）
桂林医学院引进人才科研启动基金项目(04010150001) （04010150001）
