现代食品科技2017，Vol.33Issue(10)：13-20,8.DOI:10.13982/j.mfst.1673-9078.2017.10.003
GH78家族真菌α-L-鼠李糖苷酶分子系统进化关系分析
Analysis of Evolutionary Relationship of GH78 Fungal α-L-Rhamnosidase
摘要
Abstract
In order to analyze the conserved regions and the evolutionary relationship of fungal α-L-rhamnosidase in glucoside hydrolase family 78 (GH78),87 sequences of GH78 fungal α-L-rhamnosidases were collected and compared by ClustalW2 software.In addition,the conserved amino acid sites and the common conserved regions of GH78 fungal α-L-rhamnosidases were obtained by Block Maker.Meanwhile,the signal peptide was predicted by SignalP 4.1 Server.Besides,the genetic distance and the phylogenetic tree of 87 sequences were constructed by MEGA6.0 software.Although the amino acid sequences of fungal α-L-rhamnosidases had various lengths,all contained three conserved regions,including AHGWSTGPTY,VTLDTGQNVAG and NELSIPTDGAKRD,as well as a number of conserved amino acid sites.Moreover,29 sequences contained signal peptides.The genetic distance between the genera was from 0.7 to 48.5,and the genetic distance of interspecies was from 0.4 to 48.5.Interestingly,it was found that the phylogenetic tree of the phylogenetic system was not clustered by the source of the strain,but on whether the extracellular enzyme was clustered.In this study,the conserved region and evolutionary relationships of GH78 α-L-rhamnosidases were analyzed,which laid the foundation for engineering modification of α-L-rhamnosidase.关键词
α-L-鼠李糖苷酶/保守区/分子系统进化/进化树Key words
α-L-rhamnosidase/conserved domains/evolutionary relationship/phylogenetic tree引用本文复制引用
巩建业,吴喆瑜,李利君,倪辉..GH78家族真菌α-L-鼠李糖苷酶分子系统进化关系分析[J].现代食品科技,2017,33(10):13-20,8.基金项目
国家自然科学基金资助项目(31371751) （31371751）
福建省农业高校产学合作重大项目(2014N5010) （2014N5010）
