现代食品科技2017，Vol.33Issue(10)：37-44,8.DOI:10.13982/j.mfst.1673-9078.2017.10.006
肠炎沙门氏菌和哈瓦那沙门氏菌的耐药性及耐药基因分析
Analysis of Antibiotic Resistance and Related Genes of Salmonella enteritidis and Salmonella havana
摘要
Abstract
The drug susceptibility and the related resistance genes of 17 strains of salmonella enteritidis and 11 strains of salmonella Havana had been studied in detection by Xinjiang Urumqi part of the farmer's market,in order to ensure food safety,better understanding the development of drug resistance and the ways to spread.The drug sensitivity of Salmonella was evaluated by agar dilution method.In addition,PCR and gene sequencing were used to detect the presence of mutations in the quinolone resistancedetermining region (QRDR) and plasmid-mediated quinolone resistance genes.The susceptibility rates of these 17 Salmonella enteritidis isolates were 100％ to ciprofloxacin and cefoxitin,these Salmonella isolates were 94.1％,these nalidixic acid and ceftriaxone isolates were 17.6％,and the gene detection rate of qnrB was 64.7％,17 strains of Salmonella enteritidis GyrA gene mutation,the main mutation type Asp87Tyr;The drug resistance rates of 11 Salmonella havana isolates totrimethoprim,chloromycetin,sulfadimethisoxazol,sulfamethoxazole/trimethoprim,nalidixic acid,cefoxitin were 100％,63.6％,36.4％,18.2％,9.1％,9.1％ and the sensitive rate of ampicillin,amoxicillin/clavulanic acid was 100％,the detection rate of qnrB,qnrS is 9.1％,gene mutation type of 11 strains of Salmonella Havana ParC is Thr57Ser.Salmonella enteritidis and Salmonella havana drug resistance is serious in Xinjiang Urumqi.There should be concerns about the serious case of resistance to antimicrobial in Salmonella.In addition,the quinolone resistancedetermining region (QRDR) mutations and the plasmid-mediated quinolone resistance genes may affect the antimicrobial resistance of Salmonella enteritidis and Salmonella havana.关键词
肠炎沙门氏菌/哈瓦那沙门氏菌/耐药性/耐药基因/聚合酶链式反应Key words
Salmonella enteritidis/Salmonella havana/drug resistance/drug resistance gene/polymerase chain reaction (PCR)引用本文复制引用
武运,吴浩天,宋生建,尹明远,田歌,马文瑞,王威,张亚南,古丽娜孜..肠炎沙门氏菌和哈瓦那沙门氏菌的耐药性及耐药基因分析[J].现代食品科技,2017,33(10):37-44,8.基金项目
新疆维吾尔自治区“十三五”重大专项项目“新疆民族特色食品品质分析及技术标准研究”(2016A01001-5) （2016A01001-5）
