现代食品科技2017，Vol.33Issue(10)：77-82,6.DOI:10.13982/j.mfst.1673-9078.2017.10.012
金银花挥发油及残渣浸膏的抗菌活性研究
Antibacterial Activity of Volatile Oil and Residue Extract from Lonicera Japonica
摘要
Abstract
Lonicerajaponica,obtained from Shandong and Guangdong,was used as the raw material in this study.The volatile oil was separated by CO2 supercritical fluid extraction,and afterwards the water-soluble and the water-insoluble subfraction were prepared from the residue after volatile oil isolation.The chemical composition of volatile oil was analyzed by GC-MS and high performance liquid chromatography was used to determine the content of chlorogenic acid and galuteolin in residue extract of Lonicerajaponica.The anti-bacterial activities of volatile oil and extract were investigated by disk diffusion assay and minimum inhibitory concentration (MIC).The main components of Lonicerajaponica volatile oil were fumaronitrile (61.5％) and amyl acetate (37.7％),and the content of lorogenic acid and galuteolin in water-soluble residue extract from Shandong province was the highest among the 4 residue extracts.The Lonicerajaponica volatile oil and subfractions of ethanol extract exhibited different inhibitory effects on the six pathogens.The antimicrobial effect of the two extracts on gram-positive bacteria was better than that of gram-negative bacteria.The antibacterial effect of the volatile oil in Lonicera japonica from Shandong was the strongest with the MIC of 64 μL/mL and the diameter of inhibition zone of 9.3 mm.Meanwhile,the water-soluble subfraction of Lonicerajaponica from Guangdong showed a significantly high antibacterial effect on bacillus subtilis with the diameter of inhibition zones above 15mm and MIC value of 16 pL/mL.There was no significant difference in the antibacterial activity of the extract in Lonicerajaponica from Guangdong and Shandong.关键词
金银花/挥发油/浸膏/抗菌活性Key words
Lonicera japonica/volatile oil/extract/antibacterial activity引用本文复制引用
阮之阳,王兆梅,黎嘉嘉,韦希琼..金银花挥发油及残渣浸膏的抗菌活性研究[J].现代食品科技,2017,33(10):77-82,6.基金项目
国家自然科学基金资助项目(E8160440、B5130810) （E8160440、B5130810）
