现代食品科技2017，Vol.33Issue(10)：117-126,10.DOI:10.13982/j.mfst.1673-9078.2017.10.018
葛根总黄酮与葛根素提取工艺优化及增强结肠炎大鼠抗氧化能力
Optimization of Extraction Technology of Total Flavonoids and Puerarin in Rdix Puerariae and their Enhanced Antioxidant Capacity in Colitis Rats
摘要
Abstract
Based on the traditional ethanol heat reflux method to extract puerarin and flavone,the quadratic-orthogonal-rotation -combination design was used to optimize the extraction of total flavonoids and puerarin from Rdix Puerariae.Regression models were established for the yield of total flavonoid (Y1) and the yield ofpuerarin (Y2) with the other four test factors (including extraction temperature (x1),reflux time (x2),ethanol concentration (x3) and ratio of material to liquid (x4),respectively.The regression model showed that,the most important two factors that affected the total flavonoids yield and puerarin yield from Radix Puerariae were:extraction temperature (x1) and ethanol concentration (x3).The optimal technical conditions were:extracting at 85 ℃ for 55 min,reflux time 55 min,ethanol concentration of 30％,solid-to-liquid ratio of 1∶17.5.Under these conditions,the extraction rate of total flavone from Radix Puerariae was 41.07 mg/g,the extraction ofpuerarin rate could be 16.50 mg/g,and the optimized extraction technology of Radix Puerariae was stable and feasible.The results showed that the Rdix Puerariae extractive had a better effective in recovering serum catalase (CAT),Cu/Zn superoxide dismutase (SOD),and phospholipid hydroperoxide glutathione peroxidase (GSH-px) levels as well as antioxidant capacity in colitis rats as compared with pure puerarin.关键词
葛根/总黄酮/葛根素/二次正交旋转组合设计/结肠炎/抗氧化Key words
Radix Puerariae/total flavone/puerarin/quadratic-orthogonal-rotation-combination design/colitis/anti-oxidation引用本文复制引用
吴轶,刘文惠,黄芳,鄢雅丽,欧阳克氙,刘建平,周世成,阮征..葛根总黄酮与葛根素提取工艺优化及增强结肠炎大鼠抗氧化能力[J].现代食品科技,2017,33(10):117-126,10.基金项目
江西省青年科学家项目(20153BCB23026) （20153BCB23026）
食品科学与技术国家重点实验室自由探索项目(SKLF-ZZB-201515) （SKLF-ZZB-201515）
