现代食品科技2017，Vol.33Issue(10)：245-253,177,10.DOI:10.13982/j.mfst.1673-9078.2017.10.034
QuEChERS-液相色谱-串联质谱法测定蔬菜中的56种农药残留
Determination of the 56 Pesticide Residues in Vegetables by QuEChERS-ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
A new method for analysis of 56 pesticide residues in vegetables using modified QuEChERS-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established to solve the technical difficulties of complex components,interference and low concentration.Vegetable samples were pretreated bymodified QuEChERS,which was extracted with acetonitrile containing 1％ (V/V) acetic acid,and then salted out with sodium chloride and anhydrous sodium.purified with primary secondary amine,samples was analyzed by HPLC-MS/MS.The extract was separated on a C 18 column (4.6× 100 mm,2.6 μm) using acetonitrile and aqueous solution as the mobile phase (containing 5 mM ammonium acetate and 1％ (V/V) acetic acid),ionized in electrospray ion (ESI+) mode and then analyzed in multiple-reaction monitoring (MRM) mode.The results showed that the 56 pesticide residues showed good linear correlation in the range of 2.0 μg/L to 100 μg/L with the correlation coefficients over 0.99.The limits of detection were less than 0.01 mg/kg,and the recoveries were ranged of 65.8％～114.0％ at three spiked levels in vegetables with the relative standard deviations of0.1％～14.5％.This proposed method is rapid,simple,low-cost and effective,which can be applied to rapid screening and analysis of pesticide residues in vegetables for the risk assessments of quality and safety.关键词
QuEChERS/农药多残留/高效液相色谱串联质谱法/多反应监测Key words
QuEChERS/pesticide multi-residues/performance liquid chromatography-tandem mass spectrometry/multiple reaction monitoring引用本文复制引用
李欣,孙素群,张卫锋,陈威..QuEChERS-液相色谱-串联质谱法测定蔬菜中的56种农药残留[J].现代食品科技,2017,33(10):245-253,177,10.基金项目
2013年广东省科技计划项目(2013B020311005) （2013B020311005）
2015年广东省优秀青年教师培养项目(YQ2015188) （YQ2015188）
