现代食品科技2017，Vol.33Issue(10)：262-267,6.DOI:10.13982/j.mfst.1673-9078.2017.10.036
QuEChERS-液相色谱串联质谱法快速检测猕猴桃中的氯吡脲及其代谢产物
Determination of Forchlorfenuron and Its Metabolites in Kiwifruit Using QuEChERS-LC-MS/MS
摘要
Abstract
Forchlorfenuron (1-(2-chloro-4-pyridyl)-3-phenylurea) is a plant growth regulator,which mainly be used to increase the size and weight of kiwifruit.The standards of forchlorfenuron metabolites were obtained by chemosynthesis and forchlorfenuron and its metabolites (4-hydroxyphenyl-forchlorfenuron,3-hydroxyphenyl-forchlorfenuron,forchlorfenuron-4-O-β-D-glucoside and forchlorfenuron-3-O-β-D-glucoside) in kiwifruit were simultaneously determined by LC-MS/MS with ESI+ under the mode of multiple reaction monitoring (MRM) in this study.The pre-treatment of sample was improved by optimizing PSA addition with acetonitrile as extraction solvent for extraction of clopidourea residue.The results showed that forchlorfenuron and its metabolites had a good linear correlation within a certain concentration range,the relative standard deviations were below 7.1％ with a good repeatability,the limit of detection (LOD) was 1～3 μg/L with a limit of quantification (LOQ) of 5～10 μg/L,and recoveris were between 87％ and 108％ with the PSA addition of 400 mg/15 mL.Therefore,QuEChERS-LC-MS/MS was sensitive and accurate,which could be used for simultaneous determination of forchlorfenuron and its metabolites in kiwifruit.关键词
猕猴桃/氯吡脲/代谢产物/检测Key words
kiwifruit/forchlorfenuron/metabolites/determination/safety assessment引用本文复制引用
张志伟,高振红,袁亚红,王媛,岳田利..QuEChERS-液相色谱串联质谱法快速检测猕猴桃中的氯吡脲及其代谢产物[J].现代食品科技,2017,33(10):262-267,6.基金项目
国家农产品质量安全风险评估项目(GJFP201701403、GJFP201700301、GJFP2016014) （GJFP201701403、GJFP201700301、GJFP2016014）
陕西省科技统筹创新工程项目(2016KTCQ03-12) （2016KTCQ03-12）
