肉类研究2017，Vol.31Issue(9)：63-68,6.DOI:10.7506/rlyj1001-8123-201709011
超高效液相色谱-串联质谱法测定动物源性食品中甲基睾丸酮残留量
Determination of Methyl Testosterone Residues in Foods of Animal Origin by Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
An efficient method for the determination of methyl testosterone residues in foods of animal origin with low matrix effect and high recovery was presented by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS).Samples were extracted with acetonitrile,and the extract was cleaned up on a C18 solid phase extraction column (SPE-C18),concentrated by nitrogen gas blowing,detected by UPLC-MS-MS and quantitated by external standard method.The calibration curve for methyl testosterone exhibited a good linearity with R2 higher than 0.998.The limit of detection (LOD) was 0.3 μg/kg,and the limit of quantification (LOQ) was 1.0 μg/kg.The recoveries of methyl testosterone in spiked samples were in the range of 70.60％-112.41％ at spiked levels of 1.0,2.0,10.0 and 40.0 μg/kg,with relative standard deviations (RSD) between 4.33％ to 9.26％.This method was simple,low-cost,sensitive and suitable for the batch determination of methyl testosterone residues in foods of animal origin.关键词
甲基睾丸酮/超高效液相色谱-串联质谱/动物源性食品/固相萃取Key words
methyl testosterone/ultra performance liquid chromatography-tandem mass spectrometry/foods of animal origin/solid phase extraction分类
化学化工引用本文复制引用
周迎春,王林裴,刘少博,华向美,刘会凌,彭新然..超高效液相色谱-串联质谱法测定动物源性食品中甲基睾丸酮残留量[J].肉类研究,2017,31(9):63-68,6.基金项目
河南出入境检验检疫局2017年进出口肉类口岸新增检测项目(20170017) （20170017）
