肉类研究2017，Vol.31Issue(9)：78-82,5.DOI:10.7506/rlyj1001-8123-201709013
基于核酸适配体的肉制品中污染物检测技术研究进展
Progress in Aptamer-Based Detection of Contaminants in Meat Products
摘要
Abstract
Nucleic acid aptamers are oligonucleotide fragments consisting of more than a dozen or dozens of nucleotides that are capable of specifically binding to a target substance obtained by in vitro screening techniques.Aptamers are small molecules and are easily synthesized and modified in vitro.The chemical stability of aptamers is good and does not depend on the biological or cellular environment.Aptamers,also known as "chemical antibodies",have the characteristics of diverse spatial structure and a wide range of target molecules and specifically recognize metal ions,organic small molecules,biological molecules,and even cells and microorganisms.This paper summarizes the recent literature regarding the binding characteristics of aptamers ligand and the existing methods for screening aptamers,and outlines the applications of aptamers in the detection of illegal additives,metal ions,pathogenic microorganisms and biological toxins in meat products.The prospects for future applications of aptamers are discussed as well.关键词
肉制品/核酸适配体/配体指数富集系统进化技术/制备/分析/应用Key words
meat products/nucleic acid aptamers/systematic evolution of ligands by exponential enrichment (SELEX)/preparation/analysis/application分类
轻工纺织引用本文复制引用
李永波,张巍,张翠侠,张涛,张雅伦,周巍,张岩..基于核酸适配体的肉制品中污染物检测技术研究进展[J].肉类研究,2017,31(9):78-82,5.基金项目
科技部公益性行业科研专项(201510208-08) （201510208-08）
国家自然科学基金青年科学基金项目(31401584) （31401584）
河北省食品药品监督管理局科技计划项目(QN2015002) （QN2015002）
国家重大科学仪器设备开发专项(2012YQ03011110) （2012YQ03011110）
