食品工业科技2017，Vol.38Issue(19)：11-20,10.DOI:10.13386/j.issn1002-0306.2017.19.003
紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用
Inhibitory effects of Zijuan tea(Camellia sinensis var.kitamura)extracts on angiotensin converting enzyme,α-amylase and pancreatic lipase in vitro
赵瑜 1周家春 1张靖伟 1张宛昕 1赵黎明 1蒋丽华1
作者信息
- 1. 华东理工大学,生物反应器工程国家重点实验室,发酵工业分离提取技术研发中心,上海200237
- 折叠
摘要
Abstract
Objective:To explore whether the extracts of Zijuan tea has the potential to anti-hypertensive,anti-hyperglycemic and anti-obesity.Methods:The crude polyphenol fraction (defined as ZTE1)was extracted from Zijuan tea.ZTE1 was then separated to the precipitated (defined as ZTE2) and the soluble (defined as ZTE3) with ethyl acetate.Subsequently,delphinidin-3-0-β-D-(6-(E)-p-coumaroyl) galactopyranoside (AN3) and cyanidin-3-O-β-D-(6-(E)-p-coumaroyl) galactopyranoside(AN3)were purified from ZTE2.The inhibitory activities of the three crude fractions and two anthocyanins towards angiotensin converting enzyme (ACE),α-amylase and pancreatic lipase were evaluated comparing with epigallocatechin gallate(EGCG).The inhibitory type of AN3 and AN4 towards ACE,α-amylase and pancreatic lipase was also explored.Results:All the Zijuan tea extracts had inhibitory activities towards the three enzymes.The inhibitory activities of ZTE3towards the three enzymes,ZTE1 towards α-amylase and pancreatic lipase,ZTE2 towards α-amylase were stronger than EGCG.Furthermore,ZTE3 had the highest inhibitory acitivity against ACE,whereas ZTE2 and ZTE1 had the highest inhibitory acitivity against α-amylase and pancreatic lipase,respectively.And the inhibitory activity of AN3 towards α-amylase was not significantly different to EGCG.The inhibitory activity of AN3 was higher than AN4 towards all the three enzymes.In addition,AN3 and AN4 were noncompetitive inhibitors of ACE,α-amylase and pancreatic lipase with Ki values of 0.33,0.37 mmol/L,0.39,0.41 mg/mL and 0.22,0.78 mg/mL.Conclusion:The extracts of Zijuan tea has the potential to anti-hypertensive,anti-hyperglycemic and anti-obesity.关键词
紫娟茶提取物/总酚/花色苷/茶多酚/血管紧张素转换酶/α-淀粉酶/胰脂肪酶Key words
Zijuan tea extracts/total phenols/anthocyanin/tea polyphenol/angiotensin converting enzyme/α-amylase/pancreatic lipase分类
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赵瑜,周家春,张靖伟,张宛昕,赵黎明,蒋丽华..紫娟茶提取物对血管紧张素转换酶、α-淀粉酶和胰脂肪酶的体外抑制作用[J].食品工业科技,2017,38(19):11-20,10.
