食品工业科技2017，Vol.38Issue(19)：67-73,7.DOI:10.13386/j.issn1002-0306.2017.19.013
基于RAW264.7细胞模型的不同茶类抗炎功能特性
Anti-inflammatory activities of six types of tea in LPS-induced RAW264.7 cells
摘要
Abstract
According to the classical processing technology,tea can be categorised into six types:green tea,white tea,yellow tea,oolong tea,puer tea,black tea.This study aimed to investigate anti-inflammatory property of six types of tea,including Yunnan Daye(green tea,black tea,and puer tea),Fuding Dabai (white tea),Junshan Yinzhen (yellow tea),and Jin Guanyin (oolong tea).Nitric oxide (NO)-scavenging assay and LPS-induced RAW264.7 cells model were used to assess the anti-inflammatory activity of water extract of six types of tea.The results showed that,among six types of tea,green,white,and yellow tea displayed stronger NO-scavenging capacity with IC50 values ranging from 99.27 to 104.83 μg/mL.Compared with other five types of tea,yellow tea exhibited significant anti-inflammatory activity against LPS-induced RAW264.7 cells.The IC50 values of yellow tea for NO-suppressing effect was 398.13 μg/mL,and the inhibition rate for tumor necrosis factor-α(TNF-α)and interleukin-6 (IL-6) reached 69.25％ and 87.68％,respectively,at a concentration of 500 μg/mL.Compared with LPS-induced group,treatment with water extract of yellow tea significantly down-regulated the expression of iNOS mRNA and decreased iNOS protein level.Correlation analysis showed that the content of tea polyphenols had a significant negative correlation with the IC50 values of NO-scavenging and NO-suppressing,TNF-α and IL-6 productions.In conclusion,all six types of tea possessed anti-inflammatory property.Among six types of tea,yellow tea exerted the strongest inhibitory effect on production of pro-inflammatory mediators(NO,TNF-α,and IL-6),and could reduce NO production through down-regulating the expression of iNOS both in transcriptional and translational level.关键词
六种类茶/RAW264.7细胞/抗炎功效/炎症介质Key words
six types of tea/RAW264.7 cell/anti-inflammatory activity/inflammatory mediators分类
轻工纺织引用本文复制引用
李晓飞,高雄,林晓蓉,张媛媛,李斌,陈忠正..基于RAW264.7细胞模型的不同茶类抗炎功能特性[J].食品工业科技,2017,38(19):67-73,7.基金项目
现代农业产业技术体系建设专项资金(CARS-23) （CARS-23）
广东省科技计划项目(2012A020602040). （2012A020602040）
