食品工业科技2017，Vol.38Issue(19)：100-104,5.DOI:10.13386/j.issn1002-0306.2017.19.019
一株琼胶降解菌的筛选、鉴定及粗酶学性质
Isolation, identification and enzymology properties of an agarase-producing strain
摘要
Abstract
An agar-decomposing strain was isolated from Qingdao offshore deposits.The optimal fermentation time was 26 h,transparent zones appeared after staining with lugol's iodine.The bacteria was gram-negative and classified in the genus Acinetobacter,named as Acinetobacter sp.LXK.By studying the primary properties of agarase and study showed that:the optimal pH and temperature for enzymolysis were pH8.0 and 40 ℃ respectively,the optimum concentration of substrate was 0.5％,the enzyme was activate by K+,Ca2+ and inhabited by Fe2+,Mn2+,Ba2+.关键词
琼胶酶/海洋细菌/筛选/鉴定Key words
agarase/marine bacteria/screening/identification分类
轻工纺织引用本文复制引用
刘兴凯,朱丹,周薪,房玲,郭爽,郭瑞,程凡升..一株琼胶降解菌的筛选、鉴定及粗酶学性质[J].食品工业科技,2017,38(19):100-104,5.基金项目
国家自然科学基金项目(31301438,31501331) （31301438,31501331）
山东省优秀中青年科学家奖励基金(BS2013SW034) （BS2013SW034）
校高层次人才启动基金(1113321) （1113321）
青岛农业大学应用型人才培养特色名校建设工程大学生科技创新项目. （）
