食品工业科技2017，Vol.38Issue(19)：142-147,6.DOI:10.13386/j.issn1002-0306.2017.19.027
金鲳鱼鱼皮酶溶性胶原蛋白的提取工艺优化及基本特性
Optimization of extraction process and basic properties for pepsin-soluble collagen from Golden pompano skin
摘要
Abstract
To improve the utilization rate of fish skin in processing by-product of Golden pompano,this paper used the response surface Box-Behnken method to optimize the extraction process of pepsin-soluble collagen (PSC) from the skin of Golden pompano.Based on the previous single factor experiment,the model was established by three factors (enzyme concentration,liquid-to-solid ratio and extraction time).In addition,the basic properties of PSC were characterized and compared with acid-soluble collagen(ASC)by Sodium Dodecyl Sulfate Poly Acrylamide Gel Electrophoresis (SDS-PAGE),Attenuated Total Reflectance Fourier Transform Infrared spectroscopy (ATR-FTIR) and Differential Scanning Calorimeter (DSC).The results showed that the optimum extraction conditions were 1.5％ enzyme concentration,23∶1 mL/g liquid-to-solid ratio,and 27.5 h extraction time.Under this condition,the actual yield of collagen of Golden pompano skin was 64.68％,which was close to the predicted value(65.17％).Moreover,the PSC obtained in this experiment had higher denaturation temperature (30.44 ℃)and preliminarily identified as type Ⅰ collagen,which was similar to the electrophoretic and infrared peaks of ASC.The technical reference for the extraction of PSC from Golden pompano skin was given in this research.关键词
金鲳鱼鱼皮/胶原蛋白/响应面法/提取工艺Key words
Golden pompano skin/collagen/response surface methodology/extraction process分类
轻工纺织引用本文复制引用
廖伟,夏光华,王佳媚,杨晋坤,李永成,申铉日..金鲳鱼鱼皮酶溶性胶原蛋白的提取工艺优化及基本特性[J].食品工业科技,2017,38(19):142-147,6.基金项目
海南省自然基金(317038) （317038）
海南省重点研发计划(ZDYF2017104) （ZDYF2017104）
海南大学科研启动基金资助项目(kyqd1662). （kyqd1662）
