食品工业科技2017，Vol.38Issue(19)：172-176,183,6.DOI:10.13386/j.issn1002-0306.2017.19.032
鳞柄小奥德蘑多糖提取工艺的优化及抗氧化活性
Extraction and antioxidant activity of polysaccharides from Oudemansiella furfuracea
摘要
Abstract
To investigate the optimum extracting process of polysaccharides from Oudemansiella furfuracea and its antioxidant activity in vitro.Extraction process of polysaccharides from Oudemansiella furfuracea was optimised by orthogonal test.And the concentration of total sugar and reducing sugar in water extraction was determined by anthrone-sulfuric acid method and DNS method,respectively.The antioxidant properties were tested by prussian blue method,salicylic acid method and pyrogallol method,respectively.The results showed that the impact sequence of the factors on the extraction rate of polysaccharide from Oudemansiella furfuracea was as follows:solid-liquid ratio ＞ extraction time ＞ extraction temperature ＞ extraction times.The optimal extraction condition was solid-liquid ratio of 1∶40,extraction time of 1 h,extraction temperature of 80 ℃,and extracted three times,and under this conditions,the extraction rates of polysaccharide from Oudemansiella furfuracea was 8.84％.In addition,crude polysaccharides from Oudemansiellafurfuracea could obviously scavenge hydroxyl radicals in a dose-dependent manner(p ＜ 0.05),and at the concentration of 4 mg/mL,crude polysaccharides was observed to possess strong free radical-scavenging effects against scavenge hydroxyl radicals,with a value of 48.94％ ± 12.32％.In addition,crude polysaccharides from Oudemansiella furfuracea had a certain reducing power lower and its scavenging effects on superoxide anion radical was ineffective.So crude polysaccharides from Oudemansiella furfuracea had certain antioxidant activity and could be used for polysaccharide drugs and health food.关键词
鳞柄小奥德蘑多糖/正交设计/抗氧化能力Key words
polysaccharide from Oudemansiella furfuracea/orthogonal test/antioxidant activity分类
轻工纺织引用本文复制引用
王晶,费爽,孟煜晗,孙天玉,侯晓强,付亚娟,杜娟,吴智艳..鳞柄小奥德蘑多糖提取工艺的优化及抗氧化活性[J].食品工业科技,2017,38(19):172-176,183,6.基金项目
河北省高等学校科学技术研究青年基金项目(QN2016019) （QN2016019）
河北省高校食药用菌应用技术研发中心项目(YF201411-321) （YF201411-321）
河北省高等学校遗传学重点发展学科项目(201221) （201221）
廊坊师范学院微生物学重点学科项目(201501) （201501）
廊坊师范学院大学生创新训练计划项目(201510100036). （201510100036）
