食品工业科技2017，Vol.38Issue(19)：240-244,5.DOI:10.13386/j.issn1002-0306.2017.19.044
黑果腺肋花楸原花青素的纯化及表儿茶素和原花青素B2含量测定
Purification of black chokeberry procyanidins and determination of epicatechin and procyanidins B2 by HPLC
摘要
Abstract
The adsorption and desorption properties of procyanidins from black chokeberry with AB-8 macroporous resins was studied.And the HPLC method was used to determine the content of epicatechin and procyanidins B2 in procyanidins purification.The results showed that,procyanidins concentration of the sample solution was 4.0 mg/mL,adsorption flow rate was 2 BV/h,the desorption concentration of ethanol was 50％.Under the proposed conditions,the purity of procyanidins content from black chokeberry was 14.29％.Chromatographic separation was performed on the Aglient Eclipse XDB-C18,4.6 mm × 250 mm.The mobile phase was methanol(A)-water(B) with the gradient elution(0～10 min,10％ A-30％ A;10 ～20 min,30％ A-70％ A;20～30 min,70％ A-100％ A).The flow rate was 1.0 mL/min and the detective wavelength was set at 280 nm.The linear ranges of epicatechin and procyanidins B2 concentration were 0.025～0.2 mg/mL.The sample recoveries rate of the two components were 105.5％ and 99.8％ respectively.The content of epicatechin and procyanidins B2 in black chokeberry procyanidins purification were 14.26 mg/g and 22.45 mg/g,respectively.关键词
黑果腺肋花楸/AB-8大孔树脂/原花青素/高效液相色谱(HPLC)/表儿茶素/原花青素B2Key words
black chokeberry/AB-8 macroporous resins/procyanidins/high performance liquid chromatography/epicatechin/procyanidins B2分类
轻工纺织引用本文复制引用
朱月,张海平,侯亚男,安建辉,封晓茹,温馨亚,李腾,孙爱东..黑果腺肋花楸原花青素的纯化及表儿茶素和原花青素B2含量测定[J].食品工业科技,2017,38(19):240-244,5.基金项目
国家自然科学基金(31471593) （31471593）
国家重点研发计划(2016YFD0400302-4). （2016YFD0400302-4）
