食品工业科技2017,Vol.38Issue(19):260-264,5.DOI:10.13386/j.issn1002-0306.2017.19.048
硫解法和正相色谱法测定山竹壳原花青素
Determination of proanthocyanidins from mangosteen husk by thiolysis-HPLC and normal-phase HPLC
摘要
Abstract
To investigate the content of proanthocyanidins from mangosteen husk,thiolysis-HPLC was used to analyze and quantify extractable(EP) and nonextractable (NEP)proanthocyanidins,then normal-phase HPLC was applied to isolate and determinate EP in different degrees of polymerization.Proanthocyanidins from mangosteen husk mainly consisted of epicatechin,contents and mean degree of polymerization of EP and NEP were (131.69 ± 11.24),(36.92 ± 2.38) g/kg procyanidin B2/kg and (4.27 ± 0.08),(5.41 ± 0.04) respectively.Contents of B-type procyanidin dimer,trimer and tetramer from EP were (40.51 ± 8.30),(38.70 ± 8.69),(13.95 ± 3.93) g/kg.These results can provide reference for the use of proanthocyanidins from mangosteen husk.关键词
山竹壳/原花青素/硫解/测定Key words
Garcinia mangostana L.husk/proanthocyanidins/thiolysis/determination分类
轻工纺织引用本文复制引用
黄文烨,李星星,黄雪松..硫解法和正相色谱法测定山竹壳原花青素[J].食品工业科技,2017,38(19):260-264,5.基金项目
广东省农产品加工重点实验室开放基金(201504) (201504)
公益性行业(农业)科研专项(2013030775-5). (农业)