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硫解法和正相色谱法测定山竹壳原花青素

黄文烨 李星星 黄雪松

食品工业科技2017,Vol.38Issue(19):260-264,5.
食品工业科技2017,Vol.38Issue(19):260-264,5.DOI:10.13386/j.issn1002-0306.2017.19.048

硫解法和正相色谱法测定山竹壳原花青素

Determination of proanthocyanidins from mangosteen husk by thiolysis-HPLC and normal-phase HPLC

黄文烨 1李星星 1黄雪松1

作者信息

  • 1. 暨南大学食品科学与工程系,广东广州510632
  • 折叠

摘要

Abstract

To investigate the content of proanthocyanidins from mangosteen husk,thiolysis-HPLC was used to analyze and quantify extractable(EP) and nonextractable (NEP)proanthocyanidins,then normal-phase HPLC was applied to isolate and determinate EP in different degrees of polymerization.Proanthocyanidins from mangosteen husk mainly consisted of epicatechin,contents and mean degree of polymerization of EP and NEP were (131.69 ± 11.24),(36.92 ± 2.38) g/kg procyanidin B2/kg and (4.27 ± 0.08),(5.41 ± 0.04) respectively.Contents of B-type procyanidin dimer,trimer and tetramer from EP were (40.51 ± 8.30),(38.70 ± 8.69),(13.95 ± 3.93) g/kg.These results can provide reference for the use of proanthocyanidins from mangosteen husk.

关键词

山竹壳/原花青素/硫解/测定

Key words

Garcinia mangostana L.husk/proanthocyanidins/thiolysis/determination

分类

轻工纺织

引用本文复制引用

黄文烨,李星星,黄雪松..硫解法和正相色谱法测定山竹壳原花青素[J].食品工业科技,2017,38(19):260-264,5.

基金项目

广东省农产品加工重点实验室开放基金(201504) (201504)

公益性行业(农业)科研专项(2013030775-5). (农业)

食品工业科技

OA北大核心CSCDCSTPCD

1002-0306

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