食品工业科技2017，Vol.38Issue(20)：90-97,8.DOI:10.13386/j.issn1002-0306.2017.20.017
酶法辅助提取桂花精油工艺优化
Optimization of extraction process of Osmanthus essential oil by enzyme-assisted
摘要
Abstract
Yield of Osmanthus essential oil was to measure the improvement of Osmanthus essential oil extraction.In a single factor such as temperature,time,dosage,liquid ratio,ratio ofβ3-glucosidase,and pH on the effect of the yield of essential oil,the response surface analysis was used to optimize the enzyme treatment conditions and the establishment of a reliable multiple quadratic regression model.The results showed that when the liquid to solid ratio of 20.3,enzyme dosage 50.7 IU/g including 48.3％ β-glucosidase,in 46 ℃,pH4.6 under reflux reaction 2.7 h,yield of Osmanthus essential oil could be raised from 1.77％ to 2.75％,which was raised 55.35％.Compared with onlyβ-glucosidase used,the yield was raised 1.30 times.L-linalool,β-ionone,dihydro-β-ionone,β-ionol,y-decalactone,geraniol,perilla alcohol and nerol of the essential oil was increased by 56.33％,4.38％,21.54％,169.07％,56.52％,230.92％,157.47％,154.41％ and 16.97％ in the content of phthalate esters by GC-MS.The yield of enzyme-assisted extracting Osmanthus essential oil was improved while the content of main aroma components in Osmanthus oil could be increased and the content of harmful substances also could be reduced.Thus,the quality of the Osmanthus oil was improved.关键词
桂花精油/复合酶/水解Key words
Osmanthus oil/complex enzyme/hydrolysis分类
轻工纺织引用本文复制引用
张雪松,裴建军,赵林果,汤锋,房仙颖,解静聪..酶法辅助提取桂花精油工艺优化[J].食品工业科技,2017,38(20):90-97,8.基金项目
国家林业局林业公益性行业科研基金专项(201404601) （201404601）
江苏省生物质绿色燃料与化学品重点实验室开放基金(JSBGFC14014). （JSBGFC14014）
