食品工业科技2017，Vol.38Issue(20)：132-136,5.DOI:10.13386/j.issn1002-0306.2017.20.024
不同培养基质上阪崎克罗诺杆菌生物膜的比较
Comparison of Cronobacter sakazakii biofilm on different materials
摘要
Abstract
In order to investigate Cronobacter sakazakii.biofilm(48 h)on the surface of glass slide,polypropylene and stainless steel,the fluorescence image stacks of C.sakazakii biofilm tagged with SYTO 9/PI was assayed with hyphened technique of the program confocal laser scanning microscopy(CLSM) and COMSTAT.The result showed that the sequence from high to low of total biomass,average thickness and average diffusion distance was:stainless steel,polypropylene and glass slide,while the sequence from high to low of roughness coefficient and surface to volume was:glass slide,polypropylene and stainless steel.Live/dead ratio of C.sakazakii biofilm on the surface of glass slide was higher than polypropylene and stainless steel.Compared with strain Ⅰ and Ⅱ,the hiofilm of C.sakazakii strain ATCC 29544 had higher level of total biomass,live/dead ratio,average thickness and average diffusion distance,while roughness coefficient and surface to volume had lower level.In conclusion,C.sakazakii produced stronger biofilm on the surface of stainless steel than glass slide and polypropylene,and the biofilm forming abilitv of strain ATCC 29544 was higher than strain Ⅰ and Ⅱ isolated from food.关键词
阪崎克罗诺杆菌/生物膜/CLSM-COMSTAT/玻璃/聚丙烯/不锈钢Key words
Cronobacter sakazakii/biofilm/CLSM-COMSTAT/glass/polypropylene/stainless steel分类
轻工纺织引用本文复制引用
游雪娇,赵智颖,马连营,彭斐元,李良秋..不同培养基质上阪崎克罗诺杆菌生物膜的比较[J].食品工业科技,2017,38(20):132-136,5.基金项目
广东省科学院分析测试基金项目(Sf201502). （Sf201502）
