食品工业科技2017，Vol.38Issue(20)：190-196,203,8.DOI:10.13386/j.issn1002-0306.2017.20.034
响应面法优化红芸豆种皮花色苷的超声波辅助提取工艺及其抗氧化活性研究
Optimization of ultrasonic assisted extraction of anthocyanins from seed coat of red kidney bean(Phaseolus vulgaris L.)using response surface methodology and evaluation of its antioxidant activity
郝瑞林 1梁爱琦 1董倩倩 1范建凤2
作者信息
- 1. 忻州师范学院生物系,山西忻州034000
- 2. 忻州师范学院化学系,山西忻州034000
- 折叠
摘要
Abstract
In order to explore new anthocyanins resources,the ultrasonic assisted extraction of anthocyanins from seed coat of red kidney bean(Phaseolus vulgaris L.)was optimized by response surface methodology based on the single factor experiments and the antioxidative activitv of the crude extract was evaluated.According to the results,the optimal extraction solvent was 40％ ethonal(modified to pH1 by hydrochloric acid),and the highest extraction yield of 185.57 mg anthocyanins/100 g seed coat powder was achieved at an extraction temperature of 68 ℃,an ratio of raw material to solvent of 1 ∶ 24 (g/mL),an extraction time of 78 min and the ultrasonic power was 160 W.The results of antioxidation experiments showed that the highest scavenging capacity of anthocyanins against ABTS free radical,DPPH free radical was 68.11％,90.66％ respectively and the ferric reducing antioxidant power was in direct proportion to the anthocyanins concentration.The optimization of ultrasonic extraction of anthocyanins from seed coat of red kidney bean was reasonable and scientific,and the anthocyanins had stronger antioxidant activity.关键词
红芸豆种皮/花色苷/超声波/响应面设计/抗氧化活性Key words
seed coat of red kidney bean/anthocyanins/ultrasonic/response surface design/antioxidant activity分类
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郝瑞林,梁爱琦,董倩倩,范建凤..响应面法优化红芸豆种皮花色苷的超声波辅助提取工艺及其抗氧化活性研究[J].食品工业科技,2017,38(20):190-196,203,8.
