食品工业科技2017，Vol.38Issue(20)：312-316,335,6.DOI:10.13386/j.issn1002-0306.2017.20.057
不同产地东北林蛙卵胶膜中蛋白质和水解氨基酸含量差异比较
Comparison of protein and hydrolytic amino acids in Rana dybowskii jelly coats from different localities
摘要
Abstract
To compare the protein contents,as well as the amino acids and their contents in the Rana dybowskii jelly coat from Jingyu,Dunhua,Jiaohe,Fusong,Huadian and at different ages.Protein content was determined by Coomassie method.And the type,and the method for the determination of 21 kinds of hydrolyzed amino acids in Rana dybowskii jelly coat by high performance liquid chromatography with pre-column derivatization of Phenylisothiocyanate as derivatization reagent was established.The results showed that the pre-column derivatization reversed-phase high performance liquid chromatography with Phenylisothiocyanate as a derivatizing reagent was highly efficient,stable and reproducible,and it was suitable for the determination of amino acids in Rana dybowskii jelly coat.The contents of protein and amino acids in Rana dybowskii jelly coat from three-year-old were higher than those from two-year-old.There were significant differences in protein and amino acid contents among different origins.The highest protein,amino acid contents and the ratio of essential amino acids were found in Jiaohe three-year-old.The protein content was (174.33 ± 4.55) mg/g;the total amount of amino acids was (10.004 ± 0.20) mg/g;and the essential amino acids accounted for 24.57％.The lowest protein,amino acid contents were found in Husong two-year-old,the contents were (147.39 ± 1.09) mg/g,(6.414 ± 0.10) mg/g.The lowest ratio of essential amino acids produced in Huadian at the age of 2 forest frog film in 20.54％.The content of protein,the total amount of amino acids and the proportion of essential amino acids were 26.94 mg/g,3.59 mg/g and 4.03％ respectively.关键词
东北林蛙卵胶膜/蛋白质/氨基酸/柱前衍生/高效液相色谱Key words
Rana dybowskii jelly coat/protein/amino acids/precolumn derivatization/high performance liquid chromatography分类
轻工纺织引用本文复制引用
董蕊,马卓群,卫功庆,孙昌霞..不同产地东北林蛙卵胶膜中蛋白质和水解氨基酸含量差异比较[J].食品工业科技,2017,38(20):312-316,335,6.基金项目
长白山中国林蛙规范化配套养殖技术提升与示范基地建设(20130206027YY) （20130206027YY）
吉林省博士后启动基金. （）
