食品科学2017，Vol.38Issue(21)：30-37,8.DOI:10.7506/spkx1002-6630-201721005
羟自由基氧化对草鱼肌原纤维蛋白结构和凝胶性质的影响
Effect of Hydroxyl Radical Oxidation on Structural and Gel Properties of Myofibrillar Protein from Grass Carp
摘要
Abstract
In this paper,myofibrillar protein from grass carp was artificially oxidized in hydroxyl radical-generating system with H2O2 concentrations of 0.1,1.0,5.0 and 10.0 mmol/L,and the effect of oxidization on the structural characteristics and gel properties of myofibrillar protein was investigated.The results showed that the carbonyl content increased significantly (P ＜ 0.05) after oxidization by 111.35％ compared to the initial value when the concentration of H2O2 was 10.0 mmol/L.With the increase in H2O2 concentration,the total thiol group level,free thiol group level,and free amine content decreased significantly (P ＜ 0.05),while bityrosine content and surface hydrophobicity increased significantly (P ＜ 0.05).The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) result showed that hydroxyl radical oxidation could induce the crosslinking and aggregation of protein molecules,thereby forming aggregates with molecular mass higher than 200 ku.The above results indicated that the thiol group,tymsine and tryptophane residues of myofibrillar protein were modified after hydroxyl radical oxidation,thus leading to a significant change in protein structure.In addition,the gel properties of myofibdllar protein were also influenced by oxidation,the network structure of the gel was damaged,and the portable water in the gel was converted to free water.These changes finally induced apparent decreases in the gel strength and water-holding capacity.关键词
草鱼/肌原纤维蛋白/蛋白质氧化/结构特征/凝胶性质Key words
grass carp (Ctenopharyngodon idellus)/myofibrillar protein/protein oxidation/structural characteristics/gel properties分类
轻工纺织引用本文复制引用
李学鹏,励建荣,刘慈坤,周明言,王金厢,朱文慧,徐永霞,仪淑敏,林洪,李钰金..羟自由基氧化对草鱼肌原纤维蛋白结构和凝胶性质的影响[J].食品科学,2017,38(21):30-37,8.基金项目
国家自然科学基金面上项目(31771999) （31771999）
中国博士后科学基金项目(2015M582143) （2015M582143）
“十二五”国家科技支撑计划项目(2015BAD17B03) （2015BAD17B03）
辽宁省教育厅重点实验室基础研究项目(LZ2014047) （LZ2014047）
