食品科学2017，Vol.38Issue(21)：38-42,5.DOI:10.7506/spkx1002-6630-201721006
环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性
Preparation and Anticancer Activity of Cyclodextrin-Functionalized Curcumin-Loaded Silver Nanoparticles
摘要
Abstract
In this study,cyclodextrin-functionalized curcumin-loaded silver nanoparticles (Ag@β-CD@Cur) were prepared with silver nanomaterials and its anticancer activity was also studied.The surface morphology,particle size and stability of Ag@β-CD@Cur were detected by transmission electronic microscopy and Zetasizer Nano.Moreover,the effect of Ag@β-CD@Cur on morphological and viability changes of HepG2 cells were detected by phase-contrast microscopy and (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.Moreover,the population of apoptotic cells was detected by flow cytometric analysis.The experimental results showed that Ag@β-CD@Cur was obtained as highly pure,uniformly sized silver nanoparticles with a smooth surface.The average size of Ag@β-CD@Cur was 2 nm.Moreover,Ag@β-CD@Cur had good stability in water for 30 days.After treatment of HepG2 cells with Ag@β-CD@Cur for 24 h,apoptosis characteristics appeared such as cell swelling and cell nuclei pyknosis.At the same time,the cell viability reduced to 24％ of the control group.Further flow cyt.ometric analysis showed that the population of apoptotic cells increased from 2.5％ (control) to 51.0％.The results demonstrated that after nanoparticlization,curcumin has stronger anticancer activity,which will provide a technical means and theoretical data for curcumin nano-formulation.关键词
姜黄素/β-环糊精/银纳米粒子/制备/抗肿瘤活性Key words
curcumin/β-cyclodextrin/silver nanoparticles/preparation/anticancer activity分类
轻工纺织引用本文复制引用
陈建平,余苗,秦小明,谌素华,钟赛意..环糊精功能化修饰纳米银负载姜黄素的制备及其抗肿瘤活性[J].食品科学,2017,38(21):38-42,5.基金项目
国家自然科学基金青年科学基金项目(21602034) （21602034）
广东省自然科学基金博士启动项目(2016A30310332) （2016A30310332）
广东省公益研究与能力建设项目(2015A020209166) （2015A020209166）
广东省天然产物绿色加工与产品安全重点实验室开放基金项目(201617) （201617）
