食品科学2017，Vol.38Issue(21)：49-54,6.DOI:10.7506/spkx1002-6630-201721008
基于原子力显微镜和流变学特性的葛仙米多糖构象分析
Conformational Analysis of Polysaccharide from Nostoc sphaeroides Küitz.Based on Atomic Force Microscope and Rheological Properties
摘要
Abstract
In this paper,the single-molecule morphology of polysacchaddes extracted from Nostoc sphaeroides Kttz.was studied by atomic force microscopy.As a result,the polysaccharides were found to consist of flexible and rigid molecular chains and mainly exist in the form of flexible long chain with multi-branches,rigid chains with less or without branches and a divergent structure composed of three intertwined chains.Besides,a single helix for some of the branch chains was also found.The polysaccharides mainly existed in the conformation with abundant branches whose length was generally about 200-600 nm,the diameter of single molecules was within 1.0 nm,and the height of intertwined chains was about 1.3-2.3 nm.The rheological properties showed that the polysaccharides could form a weak gel,indicating that the polysaccharide molecules could be only slightly crosslinked.All rheologlcal properties confirmed that the polysaccharides had multiple flexible branches.关键词
原子力显微镜/葛仙米/多糖/流变学/链构象Key words
atomic force microscopy/Nostoc sphaeroides Kütz./polysaccharide/rheological properties/chain conformation分类
轻工纺织引用本文复制引用
莫开菊,程超,庄洋,陈美林,赵娜..基于原子力显微镜和流变学特性的葛仙米多糖构象分析[J].食品科学,2017,38(21):49-54,6.基金项目
国家自然科学基金面上项目(31171670) （31171670）
