食品科学2017，Vol.38Issue(21)：55-60,6.DOI:10.7506/spkx1002-6630-201721009
刺参体壁及罗非鱼皮酶促溶性胶原蛋白特性的对比研究
Comparative Studies on Characteristics of Pepsin-Soluble Collagen from Sea Cucumber (Stichopus japonicas) Body Wall and Tilapia (Oreochromis niloticus) Skin
摘要
Abstract
In this paper,the characteristics of pepsin-soluble collagen from the body wall of Stichopus japonicus (sjPSC) and the skin of Oreochromis niloticus (onPSC) were comparatively studied.The results showed that ultraviolet and Fourier transform-infrared spectroscopic measurements of onPSC and sjPSC were quite similar.Both PSCs contained mainly a-and β-chains as indicated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.The electrophoretic patterns of these two collagens under non-reducing and reducing conditions were quite similar,suggesting that reduction of disulfide bonds did not occur in these two collagens.Glycine was the dominant amino acid in both sjPSC and onPSC,accounting for approximately (344.57 ± 2.16)‰o and (353.00 ± 4.84)‰ of the total amino acid residues,respectively.More glutamic acid,aspartic acid,serine,valine,isoleucine and tyrosine were observed in sjPSC when compared with onPSC (P ＜ 0.05).However,more L-hydroxy proline,L-proline,leucine,phenylalanine,histidine and lysine were observed in onPSC (P ＜ 0.05).Peptide maps of both PSCs digested by V8 and a-chymotrypsin were completely different,suggesting some differences in their structure,especially in terms of ghtamic acid,asprtic acid and hydroxyproline residues.关键词
酶溶性胶原蛋白/刺参/罗非鱼/氨基酸/结构Key words
pepsin-soluble collagen/Stichopus japonicus/Oreochromis niloticus/amino acid/structure分类
轻工纺织引用本文复制引用
张正雨,李傲婷,王凤林,李委昆,唐越,于翠平,孙娜,吴海涛..刺参体壁及罗非鱼皮酶促溶性胶原蛋白特性的对比研究[J].食品科学,2017,38(21):55-60,6.基金项目
国家自然科学基金面上项目(31370037) （31370037）
辽宁省教育厅科学研究一般项目(L2015050) （L2015050）
