食品科学2017，Vol.38Issue(21)：165-169,5.DOI:10.7506/spkx1002-6630-201721026
乙醇对秀丽隐杆线虫寿命的影响及其分子机理
Effect of Ethanol on Caenorhabditis elegans Lifespan and Its Potential Molecular Mechanisms
摘要
Abstract
In this paper,the effect of ethanol on Caenorhabditis elegans lifespan was investigated by culturing it in a medium with different concentrations (4,40 and 400 mtol/L) of ethanol.The results showed that 400 mmol/L ethanol could significantly extend the median lifespan by 11.76％ and the mean lifespan by 15.78％ (P ＜ 0.05).The expression of lifespan-related genes (age-l,pdk-1,akt-1 and let-363) was detected using real-time quantitative polymerase chain reaction (qPCR) to explore the potential molecular mechanisms.Our results demonstrated that ethanol induced lifespan extension in C.elegans could be due to the suppressed expression of the above four genes and consequent regulation of the downstream genes in both the insulin/IGF signalling (IIS) and mammalian target of rapamycin (mTOR) pathways.关键词
秀丽隐杆线虫/长寿/乙醇/信号传导通路Key words
Caenorhabditis elegans/longevity/ethanol/signaling pathways分类
轻工纺织引用本文复制引用
王晋,王虹元,金玲玲,郭长禄,张治洲..乙醇对秀丽隐杆线虫寿命的影响及其分子机理[J].食品科学,2017,38(21):165-169,5.基金项目
国家自然科学基金面上项目(31071170) （31071170）
BIOX研究室启动资金项目(HIT0904) （HIT0904）
